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KO/KDValidated

[KO Validated] Bax Rabbit pAb (A0207)

文献引用 (270) 评论 (6) 说明书 收藏

Western blot analysis of lysates from wild type (WT) and Bax knockout (KO) 293T cells, using [KO Validated] Bax Rabbit pAb (A0207) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.

Western blot analysis of various lysates using [KO Validated] Bax Rabbit pAb (A0207) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.

Western blot analysis of lysates from Mouse brain , using [KO Validated] Bax Rabbit pAb (A0207) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.

Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using [KO Validated] Bax Rabbit pAb (A0207) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Immunofluorescence analysis of HeLa cells using [KO Validated] Bax Rabbit pAb (A0207) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HepG2 cells using [KO Validated] Bax Rabbit pAb (A0207) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of MCF7 cells using [KO Validated] Bax Rabbit pAb (A0207) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Bax Rabbit pAb (A0207) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using [KO Validated] Bax Rabbit pAb (A0207) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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货号: A0207

促销价：   ￥1260
货    期：现货产品
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详细信息

推荐稀释比
  • WB1:500 - 1:1000
  • IHC-P1:50 - 1:200
  • IF/ICC1:50 - 1:200
  • IP0.5ug-4ug antibody for 200ug-400ug extracts of whole cells
  • ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
浓度查询

请输入产品标签上的lot号，例如4000000001

反应物种
Human, Mouse, Rat
同种型
IgG
理论分子量
21kDa
实际分子量
21kDa
产品形式
Liquid
偶联物
Unconjugated
存储缓冲液
Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
阳性样本
HT-1080,Raji,293T
细胞定位
Cytoplasm,Cytoplasm,Mitochondrion membrane,Single-pass membrane protein.
纯化方式
Affinity purification

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用户验证的应用

应用及物种
  • WB (Eoma cell , Human pancreatic cancer cells , Human skin T cell lymphoma , Hut102 , HCT116Homo sapiens , Homo sapiens , Rattus norvegicus , Mus musculus , Ctenopharyngodon idellus , Gallus gallus , quails , Sus scrofa , Homo sapiens , Mus musculus , Mus musculus , Homo sapiens , Bos taurus , Rattus norvegicus , Felis catus , Capra hircus , Other , Oryctolagus cuniculus , Ctenopharyngodon , Giardia duodenalis , Eriocheir sinensis , Lampetra japonica , Rattus norvegicus , Coturnix japonica)
  • IF (Human liver cellRattus norvegicus , Rattus norvegicus , Homo sapiens , Mus musculus)
  • IHC (Homo sapiens , Rattus norvegicus , Gallus gallus , Homo sapiens , Mus musculus , Mus musculus)
  • IP (Mus musculus , Homo sapiens)
  • RT-PCR (Homo sapiens)
  • FC (Bos taurus)

客户数据及评论 (6)

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CustomerMay 022018
评分
Experiment Type
Western blot (WB)
Sample
Eoma cell
Description
Western blot analysis of extracts of Eoma cell, using antibody at 1:150 dilution.
CustomerApr 172018
评分
Experiment Type
Immunofluorescence (IF)
Sample
Human liver cell
Description
Immunofluorescence analysis of Human liver cell using antibody at 1:50 dilution. The positive signal is Golgi staining.
CustomerApr 062018
评分
Experiment Type
Western blot (WB)
Sample
Human pancreatic cancer cells
Description
Western blot analysis of extracts of Human pancreatic cancer cells, using antibody at 1:1000 dilution.
CustomerDec 082017
评分
Experiment Type
Western blot(WB)
Sample
Human skin T cell lymphoma
Description
Western blot analysis of extracts of Human skin T cell lymphoma, using antibody at 1:1000 dilution.
CustomerOct 212017
评分
Experiment Type
Western blot(WB)
Sample
Hut102
Description
Western blot analysis of extracts of Hut102 cells, using antibody at 1:1000 dilution.
CustomerJan 212014
评分
Experiment Type
Western blot(WB)
Sample
HCT116
Description
Western blot analysis of extracts of HCT116 cells, using Bax Polyclonal Antibody.

文献引用 (270)

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Publishing research using A0207? Please let us know so that we can cite the reference in this datasheet.

背景信息

BAX (also known as BCL2 Associated X, Bcl-2-Like Protein 4, Bcl2-L-4, BCL2L4) is a member of the BCL2 family of proteins that play a key role in the regulation of apoptosis in higher eukaryotes (https://www.uniprot.org/uniprot/Q07812). BAX comprises 4 Bcl-2 homology domains (BH1-BH4) and a C-terminal transmembrane domain. In healthy mammalian cells, BAX is localized to the cytoplasm through its interaction with the anti-apoptotic BL-2 family members BCL2L1/Bcl-xL . In response to apoptotic stimuli, however, BAX undergoes a conformational change that causes it to translocate to the outer mitochondrial membrane where it initiates the mitochondrial pathway of apoptosis via two potential mechanisms. Firstly, upon translocation to the outer mitochondrial membrane, BAX interacts with the mitochondrial voltage-dependent anion channel (VDAC) leading to the opening of the channel, loss of membrane potential, and the release of cytochrome c from the mitochondrion . The release of cytochrome C into the cytoplasm leads to the activation of Caspase3, initiating apoptosis. Secondly, activated BAX forms homodimers, which then assemble into oligomers on the mitochondrial outer membrane to create pores that permeabilize the mitochondrion leading to the release of cytochrome C.BAX has been shown to be involved in p53-mediated apoptosis. Expression of the human bax gene has been shown to be directly regulated by p53, and the bax promoter contains four motifs with homology to consensus p53-binding sites. Furthermore, p53 directly interacts with BAX to promote its activation.

RRID
AB_2757021
基因ID
581
Swiss Prot
Q07812
别名
BCL2 Associated X; Bcl-2-Like Protein 4; Bcl2-L-4; BCL2L4;BAX

抗原信息

免疫原
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bax (NP_620116.1).
序列
MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMF
进行序列比对
研究领域

关联靶点

以BAX为关键词搜索文献记录，得到上述基因与BAX的研究紧密相关，排序顺序根据研究相关程度由高到低决定。（所显示数字代表两者共同出现的已发表文献数量）
◀1/▶

关联领域

以BAX为关键词搜索文献记录，得到上述相关热词信息，可反映出BAX的研究趋势与热点。（关联领域与基因名称的距离代表其研究热度）

* 该数据来源于赛特新思(citexs.com)
来自于发表文献，仅供参考所用，ABclonal不承担任何责任。

暂无以BAX为关键词搜索的文献记录。

该服务由赛特新思提供，上述关联分析来自已发表文献，仅供参考所用，ABclonal不承担任何责任。

FAQ

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ABclonal公司的抗体，可以回收利用几次？

首先，一般抗体不推荐客户回收利用，抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，ABclonal公司也做过一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定。
注：我们将孵育完毕后剩余的抗体回收到离心管中置于4℃保存，效价高的抗体可至少保存1周，至少重复利用3次。

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ABclonal作为国产抗体品牌的优势？

武汉爱博泰克生物（ABclonal）科技有限公司是国产品牌，她成立于2011年，公司依托ABclonal美国波士顿抗体与蛋白研发中心、中国光谷生物城(武汉)抗体生产基地以及上海张江分子酶研发中心，凝聚了十余位来自哈佛大学、麻省理工、复旦大学、上海交大、中科院生化细胞所和武汉大学的全球知名分子和免疫学方面博士，组成我们的科学家团队，通过聚焦抗体与酶核心技术，致力于打破国际技术的垄断，将公司打造成为科研工具和诊断原料的国内领导品牌，乃至弯道超越国际巨头。 我们拥有包括兔多克隆抗体、小鼠单克隆抗体、兔单克隆抗体的生产研发平台，同时也有包括WB,IHC,IF,IP,CHIP在内的检测平台，我们对每一支自产的抗体进行了严格的检测。当然，我们部分直销地区也可以帮客户代购进口品牌的产品。同时也有抗体定制服务。ABclonal抗体优势：1，严自检，保质量；2产品多，指标全；3，价格低，货期短。注：ABclonal抗体价格体系详情见附件

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ABclonal抗体成分？

ABclonal抗体成分在平时工作当中，常会有客户咨询我们的抗体用的什么buffer进行保存，一般来说，我们的buffer的成分是：PBS含0.03%的proclin300、0.05%牛血清白蛋白、50%甘油；也有一些是PBS含0.03%的proclin300，50%甘油。防腐剂 Proclin 300活性成分主要是2-甲基-4-异噻唑啉-3-酮（MCI）和5-氯-2-甲基-4-异噻唑啉-3-酮（CMCI）。ProClin生物灭活剂能够迅速穿透细胞膜，抑制对细胞呼吸至关重要的特定酶，因此一接触微生物有机体就会立即抑制细胞活性。ProClin的多个特定毒性位点可以防止微生物产生高水平的耐药性。

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实验方案

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