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EIF2S1 Polyclonal Antibody

概述
货号: A0764
宿主: Rabbit
同种型: IgG
ABclonal:Western blot - EIF2S1 Polyclonal Antibody (A0764)
ABclonal:Immunohistochemistry - EIF2S1 Polyclonal Antibody (A0764)
ABclonal:Immunohistochemistry - EIF2S1 Polyclonal Antibody (A0764)
ABclonal:Immunohistochemistry - EIF2S1 Polyclonal Antibody (A0764)
ABclonal:Immunofluorescence - EIF2S1 Polyclonal Antibody (A0764)
Review
ABclonal: review for EIF2S1 Polyclonal Antibody(A0764)
优惠价
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背景

免疫原信息

免疫原Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human EIF2S1 (NP_004085.1).
序列MPGLSCRFYQHKFPEVEDVVMVNVRSIAEMGAYVSLLEYNNIEGMILLSELSRRRIRSINKLIRIGRNECVVVIRVDKEKGYIDLSKRRVSPEEAIKCEDKFTKSKTVYSILRHVAEVLEYTKDEQLESLFQRTAWVFDDKYKRPGYGAYDAFKHAVSDPSILDSLDLNEDEREVLINNINRRLTPQAVKIRADIEVACYGYEGIDAVKEALRAGLNCSTENMPIKINLIAPPRYVMTTTTLERTEGLSVLSQAMAVIKE
基因ID1965
Swiss ProtP05198
别名EIF2S1; EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A; eukaryotic translation initiation factor 2 subunit 1

应用

反应物种Human, Mouse, Rat
验证应用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
用户验证的应用
推荐稀释比
WB 1:500 - 1:1000
IHC 1:50 - 1:200
IF 1:50 - 1:200
理论分子量36kDa
实际分子量36kDa
存储缓冲液Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
实验方法Western blotting    
阳性样本LO2,Jurkat,MCF7,SW480,SKOV3,Mouse kidney,Mouse spleen
细胞定位Cytoplasmic granule
纯化方式Affinity purification

EIF2S1 Polyclonal Antibody 检测图片

ABclonal:Western blot - EIF2S1 Polyclonal Antibody (A0764) }

Western blot - EIF2S1 Polyclonal Antibody (A0764)

Western blot analysis of extracts of various cell lines, using EIF2S1 antibody (A0764) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - EIF2S1 Polyclonal Antibody (A0764) }

Immunohistochemistry - EIF2S1 Polyclonal Antibody (A0764)

Immunohistochemistry of paraffin-embedded rat heart using EIF2S1 antibody (A0764) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - EIF2S1 Polyclonal Antibody (A0764) }

Immunohistochemistry - EIF2S1 Polyclonal Antibody (A0764)

Immunohistochemistry of paraffin-embedded human liver cancer using EIF2S1 antibody (A0764) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - EIF2S1 Polyclonal Antibody (A0764) }

Immunohistochemistry - EIF2S1 Polyclonal Antibody (A0764)

Immunohistochemistry of paraffin-embedded mouse heart using EIF2S1 antibody (A0764) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - EIF2S1 Polyclonal Antibody (A0764) }

Immunofluorescence - EIF2S1 Polyclonal Antibody (A0764)

Immunofluorescence analysis of PC-12 cells using EIF2S1 antibody (A0764) at dilution of 1:100. Blue: DAPI for nuclear staining.

评论列表

CustomerOct 032018
Review for EIF2S1 Polyclonal Antibody(A0764)5
Cat.No
A0764
Application
Western blot (WB)
Sample
293T cells
Loading Amount
15 ug
Dilution
1:1000
Incubatition time
over night
Temperature
4 ℃
Exposure
10s
Remarks
Western blot analysis of extracts of 293T cells, using antibody at 1:1000 dilution.

More

>

Reviews List

CustomerOct 032018
Review for EIF2S1 Polyclonal Antibody(A0764)5
Cat.No
A0764
Application
Western blot (WB)
Sample
293T cells
Loading Amount
15 ug
Dilution
1:1000
Incubatition time
over night
Temperature
4 ℃
Exposure
10s
Remarks
Western blot analysis of extracts of 293T cells, using antibody at 1:1000 dilution.

More

>

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