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DDIT3 / CHOP Polyclonal Antibody

概述
货号: A0854
宿主: Rabbit
同种型: IgG
ABclonal:Western blot - DDIT3 / CHOP Polyclonal Antibody (A0854)
Review
ABclonal: review for DDIT3 / CHOP Polyclonal Antibody(A0854)
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背景

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.

免疫原信息

免疫原Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human DDIT3 / CHOP (NP_004074.2).
序列MAAESLPFSFGTLSSWELEAWYEDLQEVLSSDENGGTYVSPPGNEEEESKIFTTLDPASLAWLTEEEPEPAEVTSTSQSPHSPDSSQSSLAQEEEEEDQG
基因ID1649
Swiss ProtP35638
别名DDIT3; CEBPZ; CHOP; CHOP-10; CHOP10; GADD153; C/EBPzeta; DNA damage inducible transcript 3

应用

反应物种Human, Mouse, Rat
验证应用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
用户验证的应用
推荐稀释比
WB 1:500 - 1:2000
理论分子量19kDa/21kDa
实际分子量27kDa
存储缓冲液Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
实验方法Western blotting    
阳性样本Mouse testis,Mouse brain
细胞定位Cytoplasm,Nucleus
纯化方式Affinity purification

DDIT3 / CHOP Polyclonal Antibody 检测图片

ABclonal:Western blot - DDIT3 / CHOP Polyclonal Antibody (A0854) }

Western blot - DDIT3 / CHOP Polyclonal Antibody (A0854)

Western blot analysis of extracts of various cell lines, using DDIT3 / CHOP antibody (A0854) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 20s.

评论列表

CustomerSep 182018
Review for DDIT3 / CHOP Polyclonal Antibody(A0854 )5
Cat.No
A0854
Application
Western blot (WB)
Sample
human kidney cancer cells
Loading Amount
15 ug
Dilution
1:1000
Incubatition time
over night
Temperature
4 ℃
Exposure
30s
Remarks
Western blot analysis of extracts of human kidney cancer cells, using antibody at 1:1000 dilution.

More

>

Reviews List

CustomerSep 182018
Review for DDIT3 / CHOP Polyclonal Antibody(A0854 )5
Cat.No
A0854
Application
Western blot (WB)
Sample
human kidney cancer cells
Loading Amount
15 ug
Dilution
1:1000
Incubatition time
over night
Temperature
4 ℃
Exposure
30s
Remarks
Western blot analysis of extracts of human kidney cancer cells, using antibody at 1:1000 dilution.

More

>

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