Western blot analysis of Recombinant Mouse IL-4 Protein (RP01161), using IL4 Rabbit PolymAb® (A23926 ) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 10 ng per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time:30s.
