概述
货号: AE008
宿主: Mouse
同种型: IgG
ABclonal:Western blot - Mouse anti HA-Tag mAb (AE008)
ABclonal:Immunofluorescence - Mouse anti HA-Tag mAb (AE008)
ABclonal:Immunoprecipitation - Mouse anti HA-Tag mAb (AE008)
Review
ABclonal: review for Mouse anti HA-Tag mAb(AE008)
优惠价
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背景

免疫原信息

免疫原A synthetic peptide of YPYDVPDYA (HA-tag)-KLH
序列Email for sequence
基因ID
Swiss Prot
别名

应用

反应物种
验证应用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
用户验证的应用

WB (Human , Mouse , Pig)

IHC (Mouse)

CoIP (Oryza Sativa)

IP (Human)

推荐稀释比
WB 1:2000 - 1:5000
IF 1:50 - 1:500
IP 1:20 - 1:50
理论分子量
实际分子量57kDa
存储缓冲液Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
实验方法Western blotting    
阳性样本293T
细胞定位
纯化方式Affinity purification

Mouse anti HA-Tag mAb 检测图片

ABclonal:Western blot - Mouse anti HA-Tag mAb (AE008) }

Western blot - Mouse anti HA-Tag mAb (AE008)

Western blot analysis of over-expressed HA-tagged protein in 293T cell using HA-tag antibody (AE008) at different dilution. Each lane was loaded with 2 ug cell lysate.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - Mouse anti HA-Tag mAb (AE008) }

Immunofluorescence - Mouse anti HA-Tag mAb (AE008)

Immunofluorescence analysis of 293T cells using Mouse anti HA-Tag mAb (AE008). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - Mouse anti HA-Tag mAb (AE008) }

Immunoprecipitation - Mouse anti HA-Tag mAb (AE008)

Immunoprecipitation of over-expressed HA-tagged protein in 293T cells incubated with HA-tag antibody (AE008). A mock served as negative control and over-expressed 293T cell lysate served as positive control.

评论列表

CustomerJul 092016
Review for Mouse anti HA-Tag mAb(AE008)5
Cat.No
AE008
Application
Western blot(WB)
Sample
unknow
Loading Amount
20 ug
Dilution
1:1000
Incubatition time
over night
Temperature
4 ℃
Exposure
20s
Observed Band
unknow kDa
Remarks
The HA tag was linked with geneA and geneB respectively, the figure is protein identification of per strain after transgenosis.

More

>

Reviews List

CustomerJul 092016
Review for Mouse anti HA-Tag mAb(AE008)5
Cat.No
AE008
Application
Western blot(WB)
Sample
unknow
Loading Amount
20 ug
Dilution
1:1000
Incubatition time
over night
Temperature
4 ℃
Exposure
20s
Observed Band
unknow kDa
Remarks
The HA tag was linked with geneA and geneB respectively, the figure is protein identification of per strain after transgenosis.

More

>

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