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Mouse anti HA-Tag mAb (AE008)

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Western blot analysis of over-expressed HA-tagged protein in 293T cell using Mouse anti HA-Tag mAb (AE008) at different dilution. Each lane was loaded with 2 ug cell lysate.Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.

Western blot analysis of lysates from 293T cells, using Mouse anti HA-Tag mAb (AE008) at 1:5000 dilution.Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.

Western blot analysis of lysates from 293T cells, using Mouse anti HA-Tag mAb (AE008) at 1:10000 dilution.Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.

Western blot analysis of lysates from 293T cells, using Mouse anti HA-Tag mAb (AE008) at 1:5000 dilution.Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.

Western blot analysis of lysates from 293T cells, using Mouse anti HA-Tag mAb (AE008) at 1:10000 dilution.Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.

Immunofluorescence analysis of 293T cells transfected with HA-Tag fusion protein and untreated 293T cells use Mouse anti HA-Tag mAb (AE008) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation of over-expressed HA-tagged protein in 293T cells using HA-tag antibody (AE008). A mock served as negative control and over-expressed 293T cell lysate served as positive control.

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货号: AE008
促销价：   ￥520
货    期：在线咨询货期
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详细信息

推荐稀释比
  • WB1:2000 - 1:10000
  • IF/ICC1:50 - 1:200
  • IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
浓度查询

请输入产品标签上的lot号，例如4000000001

反应物种
Species independent
同种型
IgG1,Kappa
实际分子量
Refer to Figures
克隆号
AMC0503
产品形式
Liquid
偶联物
Unconjugated
存储缓冲液
Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
阳性样本
293T-HA(N-terminal),293T-NFAT(HA-C-terminal)
纯化方式
Affinity purification

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用户验证的应用

应用及物种
  • WB (Arabidopsis thaliana , Mus musculus , Homo sapiens , Mus musculus , Homo sapiens , Drosophila melanogaster , Other , Solanum tuberosum L. , Zea mays , M. oryzae , Sus scrofa , Serpentiformes , Penicillium oxalicum , Gallus gallus , Nicotiana tabacum L , P. oxalicum , Chlorocebus aethiops , Cyprinus carpio , Saccharomyces cerevisiae , Mycobacterium tuberculosis , Rattus norvegicus , Sus scrofa , B. subtilis)
  • IP (Zea mays , Homo sapiens , Other , Mus musculus , Nicotiana tabacum , Arabidopsis thaliana , Homo sapiens , Mus musculus , African trypanosomes , Vitis vinifera , Cyprinus carpio , Sus scrofa , Sus scrofa)
  • Co-IP (Oryza sativa , Arabidopsis thaliana , Homo sapiens , Mus musculus , Drosophila melanogaster , Other , Cucumis sativus , Homo sapiens , Gallus gallus , Sac fungi)
  • IF (Homo sapiens , Mus musculus , Serpentiformes , Sus scrofa , Cyprinus gibelio , Drosophila melanogaster)
  • ChIP (Homo sapiens , Mus musculus)
  • Other (Oryza sativa , Mus musculus)
  • ELISA (Mus musculus , Ctenopharyngodon idellus)
  • Pull-down (Mus musculus)
  • IHC (Homo sapiens , Mus musculus , Homo sapiens , Mus musculus)
  • RIP (Homo sapiens)
  • CUT&Tag (Other)
  • qPCR (Other)

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文献引用 (184)

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Publishing research using AE008? Please let us know so that we can cite the reference in this datasheet.

背景信息

Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.

RRID
AB_2770404
别名
HA;HA tag;HA-tag

抗原信息

免疫原
A synthetic peptide corresponding to HA tag.
序列
YPYDVPDYA
进行序列比对

FAQ

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ABclonal公司的抗体，可以回收利用几次？

首先，一般抗体不推荐客户回收利用，抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，ABclonal公司也做过一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定。
注：我们将孵育完毕后剩余的抗体回收到离心管中置于4℃保存，效价高的抗体可至少保存1周，至少重复利用3次。

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ABclonal作为国产抗体品牌的优势？

武汉爱博泰克生物（ABclonal）科技有限公司是国产品牌，她成立于2011年，公司依托ABclonal美国波士顿抗体与蛋白研发中心、中国光谷生物城(武汉)抗体生产基地以及上海张江分子酶研发中心，凝聚了十余位来自哈佛大学、麻省理工、复旦大学、上海交大、中科院生化细胞所和武汉大学的全球知名分子和免疫学方面博士，组成我们的科学家团队，通过聚焦抗体与酶核心技术，致力于打破国际技术的垄断，将公司打造成为科研工具和诊断原料的国内领导品牌，乃至弯道超越国际巨头。 我们拥有包括兔多克隆抗体、小鼠单克隆抗体、兔单克隆抗体的生产研发平台，同时也有包括WB,IHC,IF,IP,CHIP在内的检测平台，我们对每一支自产的抗体进行了严格的检测。当然，我们部分直销地区也可以帮客户代购进口品牌的产品。同时也有抗体定制服务。ABclonal抗体优势：1，严自检，保质量；2产品多，指标全；3，价格低，货期短。注：ABclonal抗体价格体系详情见附件

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ABclonal抗体成分？

ABclonal抗体成分在平时工作当中，常会有客户咨询我们的抗体用的什么buffer进行保存，一般来说，我们的buffer的成分是：PBS含0.03%的proclin300、0.05%牛血清白蛋白、50%甘油；也有一些是PBS含0.03%的proclin300，50%甘油。防腐剂 Proclin 300活性成分主要是2-甲基-4-异噻唑啉-3-酮（MCI）和5-氯-2-甲基-4-异噻唑啉-3-酮（CMCI）。ProClin生物灭活剂能够迅速穿透细胞膜，抑制对细胞呼吸至关重要的特定酶，因此一接触微生物有机体就会立即抑制细胞活性。ProClin的多个特定毒性位点可以防止微生物产生高水平的耐药性。

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实验方案

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