概述
货号: RK04084
宿主: Rabbit
纯化方式: Affinity purification
同种型: IgG
ABclonal:Western blot - Phospho-LMNA-S22 pAb (AP0777)

Western blot - Phospho-LMNA-S22 pAb (AP0777)

ABclonal:Immunoprecipitation - Phospho-LMNA-S22 pAb (AP0777)

Immunoprecipitation - Phospho-LMNA-S22 pAb (AP0777)

ABclonal:Western blot - Lamin A/C Polyclonal Antibody (A0249)

Western blot - Lamin A/C Polyclonal Antibody (A0249)

ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)

Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)

ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)

Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)

ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)

Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)

ABclonal:Immunofluorescence - Lamin A/C Polyclonal Antibody (A0249)

Immunofluorescence - Lamin A/C Polyclonal Antibody (A0249)

ABclonal:Immunofluorescence - Lamin A/C Polyclonal Antibody (A0249)

Immunofluorescence - Lamin A/C Polyclonal Antibody (A0249)

ABclonal:Immunofluorescence - Lamin A/C Polyclonal Antibody (A0249)

Immunofluorescence - Lamin A/C Polyclonal Antibody (A0249)

优惠价
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背景

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.

免疫原信息

免疫原A synthetic phosphorylated peptide around S22 of human LMNA (NP_005563.1).
Recombinant fusion protein containing a sequence corresponding to amino acids 403-572 of human Lamin C (NP_005563.1).
序列PLSPT
基因ID4000
Swiss ProtP02545
别名CDCD1;CDDC;CMD1A;CMT2B1;EMD2;FPL;FPLD;FPLD2;HGPS;IDC;LDP1;LFP;LGMD1B;LMN1;LMNC;LMNL1;MADA;PRO1;CDCD1;CDDC;CMD1A;CMT2B1;EMD2;FPL;FPLD;FPLD2;HGPS;IDC;LDP1;LFP;LGMD1B;LMN1;LMNC;LMNL1;PRO1

产品组成

产品组成名称验证应用反应物种
AP0777Phospho-LMNA-S22 pAbWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayHuman, Rat
A0249Lamin A/C Polyclonal AntibodyWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayHuman, Mouse, Rat

应用

理论分子量62-74kDa
实际分子量69kDa, 78kDa
存储缓冲液Store at -20℃. Avoid freeze/ thaw cycles.
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
细胞定位Nucleus,Nucleus envelope,Nucleus lamina,Nucleus speckle,nucleoplasm

Phospho-LMNA-S22 Antibody Kit 检测图片

Phospho-LMNA-S22 pAb (AP0777)
ABclonal:Western blot - Phospho-LMNA-S22 pAb (AP0777) }
Western blot - Phospho-LMNA-S22 pAb (AP0777)

Western blot analysis of extracts of various cell lines, using Phospho-LMNA-S22 antibody (AP0777) at 1:2000 dilution or LMNA antibody (A0249). HeLa cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours or treated by Paclitaxel (100 nM/ml) at 37℃ for 20 hours. C6 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours or treated by Paclitaxel (100 nM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 1s.

ABclonal:Immunoprecipitation - Phospho-LMNA-S22 pAb (AP0777) }
Immunoprecipitation - Phospho-LMNA-S22 pAb (AP0777)

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-LMNA-S22 pAb (AP0777). Western blot was performed from the immunoprecipitate using Phospho-LMNA-S22 pAb (AP0777) at a dilition of 1:1000. HeLa cells were treated by Paclitaxel (100 nM/ml) at 37℃ for 20 hours.


Lamin A/C Polyclonal Antibody (A0249)
ABclonal:Western blot - Lamin A/C Polyclonal Antibody (A0249) }
Western blot - Lamin A/C Polyclonal Antibody (A0249)

Western blot analysis of extracts of various cell lines, using Lamin A/C antibody (A0249) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours or treated by Paclitaxel (100 nM/ml) at 37℃ for 20 hours. C6 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours or treated by Paclitaxel (100 nM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249) }
Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)

Immunohistochemistry of paraffin-embedded rat brain using Lamin A/C antibody (A0249) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249) }
Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)

Immunohistochemistry of paraffin-embedded mouse kidney using Lamin A/C antibody (A0249) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249) }
Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)

Immunohistochemistry of paraffin-embedded Human gastric cancer using Lamin A/C antibody (A0249) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - Lamin A/C Polyclonal Antibody (A0249) }
Immunofluorescence - Lamin A/C Polyclonal Antibody (A0249)

Immunofluorescence analysis of HeLa cells using Lamin A/C antibody (A0249). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Lamin A/C Polyclonal Antibody (A0249) }
Immunofluorescence - Lamin A/C Polyclonal Antibody (A0249)

Confocal immunofluorescence analysis of HeLa cells using Lamin A/C Polyclonal Antibody (A0249) at dilution of 1:200. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Lamin A/C Polyclonal Antibody (A0249) }
Immunofluorescence - Lamin A/C Polyclonal Antibody (A0249)

Confocal immunofluorescence analysis of U-2 OS cells using Lamin A/C Polyclonal Antibody (A0249) at dilution of 1:200. Blue: DAPI for nuclear staining.

评论列表

CustomerApr 212017
review for Phospho-LMNA-S22 Antibody Kit (A0249)
5
CustomerFeb 222014
review for Phospho-LMNA-S22 Antibody Kit (A0249)
5
CustomerFeb 222014
review for Phospho-LMNA-S22 Antibody Kit (A0249)
5
CustomerFeb 212014
review for Phospho-LMNA-S22 Antibody Kit (A0249)
5

Reviews List

CustomerApr 212017
Review for Phospho-LMNA-S22 Antibody Kit (A0249)5
Cat.No
A0249
Application
Western blot(WB)
Sample
Primary mouse liver
Loading Amount
15 ug
Dilution
1:1000
Incubatition time
over night
Temperature
4 ℃
Exposure
30s
Observed Band
65 kDa
Remarks
Western blot analysis of extracts of mouse primary hepatocytes, using Lamin A/C (A0249) antibody 1:1000 .

More

CustomerFeb 222014
Review for Phospho-LMNA-S22 Antibody Kit (A0249)5
Cat.No
A0249
Application
Western blot(WB)
Sample
U251
Loading Amount
30 ug
Dilution
1:1000
Incubatition time
over night
Temperature
4 ℃
Exposure
15s
Observed Band
70 kDa
Remarks
Western blot analysis of extracts of U251 cells, using Lamin A/C Polyclonal Antibody.

More

CustomerFeb 222014
Review for Phospho-LMNA-S22 Antibody Kit (A0249)5
Cat.No
A0249
Application
Western blot(WB)
Sample
Human cells
Loading Amount
15 ug
Dilution
1:1000
Incubatition time
over night
Temperature
4 ℃
Exposure
15s
Observed Band
69/78 kDa
Remarks
Western blot analysis of extracts of Human cells, using Lamin A/C Polyclonal Antibody.

More

CustomerFeb 212014
Review for Phospho-LMNA-S22 Antibody Kit (A0249)5
Cat.No
A0249
Application
Western blot(WB)
Sample
Mouse splenocytes
Loading Amount
20 ug
Dilution
1:500
Incubatition time
over night
Temperature
4 ℃
Exposure
60s
Observed Band
70 kDa
Remarks
Western blot analysis of extracts of Mouse splenocytes, using Lamin A/C Polyclonal Antibody.

More

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