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This standard curve is only for demonstration purposes. A standard curve should be generated for eachassay.
No significant cross-reactivity or interference was observed.
Placed at 37°C for 3 days, the stability of the standard curve all conformto CV <10%.
* This package insert must be read in its entirety before using this product.
Mouse IL-17A Sandwich ELISA Immunoassay
| Recommended Concentration | Sample | |
|---|---|---|
| ELISA Capture | 0.25-1ug/mL | Rabbit anti-Mouse IL-17A (CAP)(Cat. No.RM17904) |
| ELISA Detection | 0.0025-0.01ug/mL | Rabbit anti-Mouse IL-17A (DET)(Cat. No.RM17905) |
| Standard | 15.63-1000pg/mL | Recombinant Mouse IL-17A Protein |
Mouse IL-17A is an approximately 40 kDa, secreted, disulfide-linked heterodimeric glycoprotein comprised of two members of the IL-17 family of cytokines, IL-17A and IL-17F . Members of this family demonstrate a structural motif termed a cysteine knot that also characterizes a large superfamily of growth factors. Although most cysteine knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links . Mouse IL-17A is synthesized as a 158 amino acid (aa) precursor that contains a 25 aa signal sequence and a 133 aa mature chain. The mature chain contains one potential site for N-linked glycosylation. Mouse IL-17F is synthesized as a 161 aa precursor with a 29 aa signal sequence and a 132 aa mature chain. Like IL-17A, the mature chain of IL-17F contains one potential site for N-linked glycosylation. IL-17A and IL-17F share 50% aa sequence homology and are syntenic, both located at the A4 region of mouse chromosome 1. Mouse IL-17A and IL-17F share 61% and 56% aa sequence identity with human IL-17A and IL-17F, respectively.
