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活性验证

Recombinant Human S100-A12 Protein (RP00009LQ)

Recombinant Human S100-A12 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at approximately 13 kDa.

Immobilized Recombinant Human S100A12 at 2 μg/mL (100 μL/well) can bind recombinant Human AGER/RAGE with a linear range of 0.1-27.25 ng/mL.

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货号: RP00009LQ
促销价:   ¥620
货    期:现货产品
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详细信息

种属
Human
表达宿主
E. coli
Calculated MW
11.42 kDa
Observed MW
13 kDa
标签
C-His
纯度
> 97% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Supplied in PBS, 25% glycerol, pH 7.4Contact us for customized product form or formulation.
描述
Recombinant Human S100-A12 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Glu92) of human S100A12 (Accession #NP_005612.1) fused with a 6×His tag at the C-terminus.
储存
Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
生物活性
Measured by its binding ability in a functional ELISA. Immobilized Human S100A12 at 2 μg/mL (100 μL/well) can bind recombinant Human AGER/RAGE with a linear range of 0.1-27.25 ng/mL.

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背景信息

The protein is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 proteins include at least 13 members which are located as a cluster on chromosome 1q21. This protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. The protein includes an antimicrobial peptide which has antibacterial activity.

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