Recombinant Human VEGFR-2/KDR/CD309 Protein (RP00084)

Recombinant Human VEGFR-2/KDR/CD309 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at approximately 120-140 kDa.

Immobilized PE anti-human CD309 (VEGFR2) Antibody at 1μg/mL (25 μL/well) can bind Human VEGFR2 with a linear range of 0.46-68.8 ng/mL.

All(2)|

货号: RP00084

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

84.11 kDa

Observed MW

120-140 kDa

标签

C-His

纯度

> 97% by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.

描述

Recombinant Human VEGFR-2/KDR/CD309 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala20-Glu764) of human VEGF R2/KDR (Accession #NP_002244.1) fused with a 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

1.Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells(HUVEC). The ED₅₀ for this effect is typically 10-50 μg/mL in the presence of 10 ng/mL recombinant human VEGF165.
2.Measured by its binding ability in a functional ELISA. Immobilized PE anti-human CD309 (VEGFR2) Antibody at 1 μg/mL (25 μL/well) can bind Human VEGFR2 with a linear range of 0.46-68.8 ng/mL.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. Mutations of this gene are implicated in infantile capillary hemangiomas.

基因ID

3791

Swiss Prot

P35968

别名

CD309;FLK1;VEGFR;VEGFR2;VEGF Receptor 2;KDR