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活性验证

Recombinant Human CSF-2/GM-CSF Protein (RP00094)

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Recombinant Human CSF-2/GM-CSF Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 20-32 kDa.

Recombinant Human CSF-2 stimulates cell proliferation of the TF‑1 human erythroleukemic cells. The ED50 for this effect is 30-140 pg/mL, corresponding to a specific activity of 7.14×106-3.33×107units/mg.

All(2)|
货号: RP00094

促销价：   ￥560
货    期：现货产品
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详细信息

种属
Human
表达宿主
HEK293 cells
Calculated MW
15.02 kDa
Observed MW
20-32 kDa
标签
C-His
纯度
> 92% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述
Recombinant Human CSF-2/GM-CSF Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ala18-Glu144) of human GM-CSF/CSF2 (Accession #NP_000749.2) fused with a 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 30-140 pg/mL, corresponding to a specific activity of 7.14×106-3.33×107units/mg.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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客户数据及评论 (1)

折叠内容
CustomerNov 122024
评分
Experiment Type
Cell-based assay
Sample
Monocytes
Description
Sorted monocytes were treated with 100 ng/mL GM-CSF (Cat. RP00094) and 20 ng/mL IL-4 (Cat. RP01597). After 7 days, the monocytes differentiated to Dendritic cells.

文献引用 (1)

折叠内容

Publishing research using RP00094? Please let us know so that we can cite the reference in this datasheet.

背景信息

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.

基因ID
1437
Swiss Prot
P04141
别名
CSF2;GMCSF
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