Active Recombinant Human ErbB-2/HER2/CD340 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 100-120 kDa.
The purity of Human HER2/ErbB2 Protein (Cat.RP00182) was greater than 90% as determined by SEC-HPLC.
Immobilized recombinant human ERBB2 at 10 ng/mL (100 µL/well) can bind Trastuzumab with a linear range of 4-20 ng/mL.
Epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. ErbB2 has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.
