Recombinant Human Serpin A3/Alpha-1-antichymotrypsin Protein (RP00194)

Recombinant Human Serpin A3/Alpha-1-antichymotrypsin Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 55-70 kDa.

All(1)|

货号: RP00194

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

45.87 kDa

Observed MW

55-70 kDa

标签

C-His

纯度

> 97% by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of 20mM HEPES,150mM NaCl, pH 8.0.Contact us for customized product form or formulation.

描述

Recombinant Human Serpin A3/Alpha-1-antichymotrypsin Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Asn26-Ala423) of human Serpin A3/AACT (Accession #NP_001076.2) fused with a 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured by its ability to inhibit chymotrypsin cleavage of a fluorogenic peptide substrate MCA-RPKPVE-Nval-WRK(Dnp)-NH2. The IC50 value is <11.5 nM.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a plasma alpha globulin glycoprotein, and is a member of serpin superfamily of the serine protease inhibitors. It is synthesized primarily in the liver and secreted as one of the most abundant serpins in plasma. SerpinA3 deficiency has been associated with liver disease, and mutations of this gene have been observed in patients with Parkinson disease and chronic obstructive pulmonary disease. Serpin A3 is a major constituent of the plaques associated with Alzheimer’s disease and an inhibitor of amyloid beta peptide degradation.

基因ID

Swiss Prot

P01011

别名

AACT; ACT; GIG24; GIG25;SERPINA3;ACT;GIG24;GIG25

研究领域