Recombinant Human Kallikrein-8/KLK8 Protein (RP00226)

Recombinant Human KLK-8/Kallikrein-8 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 35-38 kDa.

All(1)|

货号: RP00226

详细信息

种属

Human

表达宿主

HEK293 cells

描述

Recombinant Human Kallikrein-8/KLK8 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln29-Gly260) of human KLK-8/Kallikrein-8 (Accession #NP_009127.1) fused with a 6×His tag at the C-terminus.

标签

C-His

纯度

> 95% by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

生物活性

Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >44.95 pmol/min/μg.

制剂

Lyophilized from a 0.22 μm filtered solution of 20mM Tris,150mM NaCl, pH 8.0.Contact us for customized product form or formulation.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

背景信息

Kallikrein-8, also known as Neuropsin, Serine protease 19, Serine protease TADG-14, Tumor-associated differentially expressed gene 14 protein and KLK8, is a secreted protein which belongs to the peptidase S1 family and Kallikrein subfamily. It is a serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Kallikrein-8 / KLK8 is involved in skin desquamation and keratinocyte proliferation and plays a role in the secondary phase of pathogenesis following spinal cord injury. Kallikrein-8 / KLK8 is expressed at high levels in serum, ascites fluid and tumor cytosol of advanced stage ovarian cancer patients and may serve as a marker of ovarian cancer.

基因ID

11202

Swiss Prot

O60259

别名

KLK8; HNP; NP; NRPN; PRSS19; TADG14; kallikrein-8;Kallikrein 8;HNP;NP;NRPN;PRSS19;TADG14

研究领域