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活性验证

Recombinant Human ICAM-1/CD54 Protein (RP00272)

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Recombinant Human ICAM-1/CD54 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 70-80 kDa.

Immobilized APC Mouse Anti-Human CD54 at 1μg/mL (25 μL/well) can bind Human CD54 with a linear range of 0.46-7.8 ng/mL.

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货号: RP00272
促销价：   ￥560
货    期：现货产品
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详细信息

种属
Human
表达宿主
HEK293 cells
Calculated MW
50.34 kDa
Observed MW
70-80 kDa
标签
C-His
纯度
> 95% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述
Recombinant Human ICAM-1/CD54 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Gln28-Glu480) of human ICAM-1/CD54 (Accession #NP_000192.2) fused with a 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
Measured by its binding ability in a functional ELISA.Immobilized APC Mouse Anti-Human CD54 at 1μg/mL (25 μL/well) can bind Human CD54 with a linear range of 0.46-7.8 ng/mL.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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文献引用 (2)

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Publishing research using RP00272? Please let us know so that we can cite the reference in this datasheet.

背景信息

This protein is a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.

基因ID
3383
Swiss Prot
P05362
别名
BB2;CD54;P3.58;ICAM1
研究领域
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