Recombinant Human Mature TGF-beta 2 Protein (RP00452)

Recombinant Human Mature TGF-beta 2 Protein was determined by SDS-PAGE under reducing (R) and non-reducing (NR) conditions, showing bands at 13-15 kDa and 25-30 kDa, respectively.

Recombinant Human Mature TGF-beta 2 Protein inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. The ED₅₀ for this effect is 0.058-0.232 ng/mL, corresponding to a specific activity of 4.3×10⁶~1.72×10⁷ units/mg.

All(2)|

货号: RP00452

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

12.72 kDa

Observed MW

13-15 kDa

标签

No tag

纯度

> 95% by SDS-PAGE.

内毒素

< 1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.2 μm filtered solution of 4 mM HCl.Contact us for customized product form or formulation.

描述

Recombinant Human TGF-beta 2 Protein is produced by Human Cell expression system. The target protein is expressed with sequence (Ala303-Ser414) of human TGF-beta 2/TGFB2 (Accession #P61812).

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Recombinant Human Mature TGF-beta 2 Protein inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. The ED50 for this effect is 0.058-0.232 ng/mL, corresponding to a specific activity of 4.3×106~1.72×107 units/mg.

复溶

Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

背景信息

This protein belongs a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types by transducing their signal through combinations of transmembrane type I and type II receptors (TGFBR1 and TGFBR2) and their downstream effectors, the SMAD proteins. Disruption of the TGFB/SMAD pathway has been implicated in a variety of human cancers. The encoded protein is secreted and has suppressive effects of interleukin-2 dependent T-cell growth. Translocation t(1;7)(q41;p21) between this gene and HDAC9 is associated with Peters' anomaly, a congenital defect of the anterior chamber of the eye. The knockout mice lacking this gene show perinatal mortality and a wide range of developmental, including cardiac, defects. Alternatively spliced transcript variants encoding different isoforms have been identified.

基因ID

7042

Swiss Prot

P61812

别名

TGFB2;G-TSF;LDS4;TGF-beta2

研究领域