Recombinant Rat CCL5/RANTES Protein (RP00604)

Recombinant Rat CCL5/RANTES Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.showing a band at 5-14 kDa.

All(1)|

货号: RP00604

详细信息

种属

Rat

表达宿主

E. coli

Calculated MW

10 kDa

Observed MW

13 kDa

标签

N-6×His

纯度

> 95% by SDS-PAGE.

内毒素

< 1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 500mM NaCl, 2mM EDTA, pH7.4.Contact us for customized product form or formulation.

描述

Recombinant Rat CCL5/RANTES Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ser25-Ser92) of rat CCL5/RANTES (Accession #P50231) fused with an initial Met at the N-terminus and a 6×His tag at the N-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

C-C motif chemokine 5(CCL5) is a β-chemokine that plays a primary role in the inflammatory immune responseby means of its ability to attract and activate leukocytes. CCL5 is secreted by many cell types at inflammatorysites, and it exerts a wide range of activities through the receptors CCR1, CCR3, CCR4, and CCR5. Inflammatoryresponses can be impaired by the sequestration of CCL5 by the cytomegalovirus protein US28. Oligomerizationof CCL5 on glycosaminoglycans is required for CCR1mediated leukocyte adhesion and activation as well asCCL5 ’ s interaction with the chemokine CXCL4/PF4.The deposition of CCL5 on activated vascular endothelialcells is crucial for monocyte adhesion to damaged vasculature, but CCL5 oligomerization is not required for theextravasation of adherent leukocytes.CCL5 is upregulated in breast cancer and promotes tumor progressionthrough the attraction of proinflammatory macrophages in addition to its actions on tumor cells, stromal cells,and the vasculature.

基因ID

81780

Swiss Prot

P50231

别名

C-C motif chemokine 5; SIS-delta; Small-inducible cytokine A5; T-cell-specific protein RANTES;Ccl5; Scya5