Recombinant Mouse CCL3/MIP-1 alpha Protein (RP00747)

说明书收藏

Recombinant Mouse CCL3/MIP-1 alpha Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

All(1)|

货号: RP00747

详细信息

种属

Mouse

表达宿主

E. coli

Calculated MW

10.8 kDa

Observed MW

15 kDa

标签

N-6×His

纯度

> 95% by SDS-PAGE.

内毒素

< 1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.2 μm filtered solution of 20mM Tris,150mM NaCl,5%Trehalose,1mM EDTA,pH8.0.Contact us for customized product form or formulation.

描述

Recombinant Mouse CCL3/MIP-1 alpha Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ala24-Ala92) of mouse CCL3/MIP-1 alpha (Accession #P10855) fused with an initial Met at the N-terminus and a 6×His tag at the N-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

C-C Motif Chemokine 3 (MIP-1 alpha,CCL3 ) is a member of the beta or CC subfamily of chemokines and isclosely related to CCL4/MIP-1 beta. CCL3 expression can be induced in a variety of hematopoietic cells,fibroblasts, smooth muscle cells, and epithelial cells. Mature mouse CCL3 shares 73%, 91%, and 82% aminoacid sequence identity with human, rat, and cotton rat CCL3, respectively. CCL3 exerts its biological functionsthrough interactions with CCR1, CCR3, and CCR5. It is cleared from the extracellular space by internalization viathe decoy chemokine receptor D6. CCL3 promotes the chemoattraction, adhesion to activated vascularendothelium, and cellular activation of many hematopoietic cell types including activated T cells, NK cells,neutrophils, monocytes, immature dendritic cells, and eosinophils. CCL3 is also known as stem cell inhibitor(SCI) and can inhibit the proliferation of hematopoietic progenitor cells. CCL3 bioactivity contributes to tumormetastasis and the inflammatory components of viral infection, rheumatoid arthritis, and hepatitis, although italso can suppress the replication of HIV.

基因ID

20302

Swiss Prot

P10855

别名

C-C motif chemokine 3;Heparin-binding chemotaxis protein; Macrophage inflammatory protein 1-alpha;MIP1-a; chemokine (C-C motif) ligand 3; LD78a; LD78 alpha; MIP-1 alpha;Scya3