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Recombinant Human LAIR-2/CD306 Protein (RP01034)

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Recombinant Human LAIR-2/CD306 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 18-22 kDa.

All(1)|
货号: RP01034
促销价：   ￥560
货    期：现货产品
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详细信息

种属
Human
表达宿主
HEK293 cells
描述
Recombinant Human LAIR-2/CD306 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln22-Pro152) of human LAIR2 (Accession #NP_002279) fused with a 6×His tag at the C-terminus.
标签
C-His
纯度
> 90% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
生物活性
Measured by the ability of the immobilized protein to support the adhesion of HT-29 human colon adenocarcinoma cells. When 5 x 104 cells/well are added to rhLAIR-2 coated plates (50 μg/mL with 100 μL/well), approximately 50%-60% will adhere after 10 minutes at 37℃.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
储存
Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

Leukocyte-associated immunoglobulin-like receptor 2 ( LAIR2 ), also known as CD36, is a 131 amino acid protein containing one lg-like C2-type domain, making it a member of the Ig superfamily. When compared to LAIR-1, its transmembrane counterpart, it shares 83% aa identity across the signal sequence and extracellular domains, the two LAIR proteins are thought to have arisen from a common gene ancestor and appear to share similar adhesion profiles. LAIR2 is thought to be secreted and may help modulate mucosal tolerance. As a natural competitor for LAIR1, soluble LAIR2 prevents binding of human LAIR1 to collagens and LAIR1 cross-linking, thereby regulating its inhibitory potential. LAIR2 is thought to be secreted and may help modulate mucosal tolerance.

基因ID
3904
Swiss Prot
Q6ISS4
别名
LAIR2;CD306
研究领域
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