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Recombinant Mouse IFN-gamma Protein (RP01070)

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Recombinant Mouse IFN-gamma Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 55 kDa.

Immobilized recombinant Mouse IFNGR1 at 0.2μg/mL (100μL/well) can bind recombinant Mouse IFNG,the EC50 of Mouse IFNG is 2.2ng/mL.

Stimulates mouse bone marrow-derived macrophages and peritoneal macrophages differentiation into M1 Macrophages with IFN-γ. The expression of the related biological markers TNF-a, Nos2, IL-1β, and IL-6 were detected to be significantly upregulated. (Customer Feedback Data)

Recombinant Mouse IFNG protein stimulates ZBP1 gene (ISGs) expression in mouse L-929 cells. 10 ng/mL of Mouse IFNG can effectively stimulate the expression of ZBP1 gene after 24h. (Customer Feedback Data)

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货号: RP01070
促销价：   ￥380
货    期：现货产品
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详细信息

种属
Mouse
表达宿主
HEK293 cells
描述
Recombinant Mouse IFN-gamma Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Cys155) of mouse Interferon Gamma (Accession #NP_032363.1) fused with an Fc, 6×His tag at the C-terminus.
标签
C-hFc&His
纯度
> 92% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
生物活性
1.Measured by its binding ability in a functional ELISA. Immobilized recombinant Mouse IFNGR1 at 0.2 μg/mL (100 μL/well) can bind recombinant Mouse IFNG, the EC50 of Mouse IFNG is 2.2 ng/mL.
2.Recombinant Mouse IFN-gamma stimulates mouse bone marrow-derived macrophages and peritoneal macrophages differentiation into M1 Macrophages(classically activated macrophage). The expression of the related biological markers TNF-a, Nos2, IL-1β, and IL-6 was detected to be significantly upregulated.
3.Recombinant Mouse IFNG protein stimulates ZBP1 gene(ISGs) expression in mouse L-929 cells. 10 ng/mL of IFNG can effectively stimulate the expression of ZBP1 gene after 24h.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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文献引用 (2)

折叠内容

Publishing research using RP01070? Please let us know so that we can cite the reference in this datasheet.

背景信息

Interferon-gamma (IFN-gamma ) is a secreted protein which belongs to the type II interferon family. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. Interferon-gamma is a central regulator of the immune response and signals via the Janus Activated Kinase (JAK)-Signal Transducer and Activator of Transcription (STAT) pathway.

基因ID
15978
Swiss Prot
P01580
别名
IFG;IFI;IFN gamma;Interferon Gamma;IFNG
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