Recombinant Mouse Placenta growth factor/PlGF/PGF Protein (RP01080)

Recombinant Mouse Placenta growth factor/PlGF/PGF Protein was resolved with SDS PAGE under reducing (R) and non-reducing (NR) conditions ，showing single bands at 60 kDa and 120 kDa, respectively.

Immobilized Recombinant Human VEGFR1 at 500ng/mL (100 μL/well) can bind Recombinant Mouse PLGF with a linear range of 12-49 ng/mL.

All(2)|

货号: RP01080

详细信息

种属

Mouse

表达宿主

HEK293 cells

Calculated MW

44.66 kDa

Observed MW

60 kDa

标签

C-hFc&His

纯度

> 87% by SDS-PAGE.

内毒素

< 1.0 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.

描述

Recombinant Mouse Placenta growth factor/PlGF/PGF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Pro158) of mouse PLGF/PGF (Accession #NP_032853.1) fused with an Fc, 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human VEGFR1 at 500 ng/mL (100 μL/well) can bind Recombinant Mouse PLGF with a linear range of 12-49 ng/mL.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

客户数据及评论 (0)

折叠内容

背景信息

Placenta growth factor (PlGF) is a member of the PDGF/VEGF family of growth factors that share a conserved pattern of eight cysteines. Alternative splicing results in at least three human mature PlGF forms containing 131 (PlGF-1), 152 (PlGF-2), and 203 (PlGF-3) amino acids (aa) respectively. Only PlGF-2 contains a highly basic heparin-binding 21 aa insert at the C-terminus. Human PlGF-1 shares 56%, 55%, 74% and 95% aa identity with the comparable isoform of mouse, rat, canine, and equine PlGF, respectively. PlGF is mainly found as variably glycosylated, secreted, 55-60 kDa disulfide linked homodimers. Mammalian cells expressing PlGF include villous trophoblasts, decidual cells, erythroblasts, keratinocytes, and some endothelial cells. Circulating PlGF increases during pregnancy, reaching a peak in mid-gestation; this increase is attenuated in preeclampsia. However, deletion of PlGF in the mouse does not affect development or reproduction. Postnatally, mice lacking PlGF show impaired angiogenesis in response to ischemia.

基因ID

18654

Swiss Prot

P49764

别名

PGF;PLGF;PlGF2;PlGF;PGFL;SHGC-10760;PGF