Recombinant Human Osteoactivin/GPNMB Protein (RP01390)

Recombinant Human Osteoactivin/GPNMB Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 70-100kDa.

Immobilized recombinant Human GPNMB Protein at 1μg/mL (100 μL/well) can bind GPNMB Rabbit pAb with a linear range of 0.976-116.5ng/mL.

All(2)|

货号: RP01390

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

54.02 kDa

Observed MW

70-100 kDa

标签

C-His

纯度

> 95% by SDS-PAGE.

内毒素

<0.1EU/μg

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

描述

Recombinant Human Osteoactivin/GPNMB Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Pro474) of human Osteoactivin/GPNMB (Accession #NP_002501.1) fused with a 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured by its binding ability in a functional ELISA. Immobilized Human GPNMB Protein at 1 μg/mL (100 μL/well) can bind GPNMB Rabbit pAb with a linear range of 0.976-116.5 ng/mL.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

Transmembrane glycoprotein NMB (GPNMB) is also known as Transmembrane glycoprotein HGFIN, DC-HIL and Osteoactivin (OA), which belongs to the PMEL/NMB family. GPNMB contains one PKD domain.GPNMB protein acts as a downstream mediator of BMP-2 effects on osteoblast differentiation and function. GPNMB participates in bone mineralization and functions as a negative regulator of inflammation in macrophages. Osteoactivin is expressed at high levels in normal and inflammatory liver macrophages suggesting a significant role in acute liver injury. The early-phase upregulation of Osteoactivin expression in the tubular epithelium in response to renal injury might play a role in triggering renal interstitial fibrosis via activation of matrix metalloproteinase expression and collagen remodeling in rats. Osteoactivin is a protein that is expressed in aggressive human breast cancers and is capable of promoting breast cancer metastasis to bone.

基因ID

10457

Swiss Prot

Q14956-2

别名

GPNMB;HGFIN;NMB

研究领域