Recombinant Human Periostin/PN/POSTN Protein (RP01748)

Recombinant human Periostin/PN/POSTN Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 100-110 kDa.

All(1)|

货号: RP01748

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

91.80 kDa

Observed MW

100-110 kDa

标签

C-6His

内毒素

< 1EU/μg

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

描述

Recombinant Human Periostin/PN/POSTN Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asn22-Gln836) of human Periostin/PN/POSTN (Accession #NP_006466.2) fused with a 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. Immobilized Recombinant Human Periostin/OSF‑2 at 10 µg/mL (100 µL/well) induces >19% cell adhesion.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

客户数据及评论 (1)

折叠内容

CustomerOct 232024

评分
Experiment Type: Cell-based assay
Sample: triple negative breast cancer cell line
Description: Human triple negative breast cancer cell line 1833 was treated with 300 ng/mL rhPOSTN for 6 hours and samples were collected. WB results showed that the level of FAK phosphorylation in 1833 cells was up-regulated. In order to further illustrate the changes in FAK phosphorylation levels, the FAK phosphorylation inhibitor PF573228 was added to rhPOSTN treatment and also treated for 6 hours. The results of WB showed that the up-regulation of FAK phosphorylation in 1833 induced by rhPOSTN was inhibited after addition of inhibitors.

背景信息

Periostin ( POSTN ), also known as OSF2 (osteoblast specific factor 2), is a heterofunctional secreted extracellular matrix (ECM) protein comprised of four fasciclin domains that promotes cellular adhesion and movement, as well as collagen fibrillogenesis.Postn is frequently overexpressed in various types of human cancers, stimulating metastatic growth by promoting cancer cell survival, invasion and angiogenesis, and can be a useful marker to predict the behavior of cancer.

基因ID

10631

Swiss Prot

Q15063

别名

PN; OSF2; OSF-2; PDLPOSTN;POSTN

研究领域