Recombinant Human PDGF-BB Protein (RP01763)

Recombinant Human PDGF-BB Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 10-17 kDa.

Immobilized PDGF-BB (Catalog: RP01763) at 2 μg/mL (100 μL/well) can bind Human PDGFRB (Catalog: RP00126) with a linear range of 0.1-75.4 ng/mL.

Recombinant Human PDGF-BB Protein was measured in a cell proliferation assay using BALB/3T3 mouse fibroblasts. The ED₅₀ for this effect is 2.09-8.36 ng/mL, corresponding to a specific activity of 1.20×10⁵~4.78×10⁵ units/mg.

All(3)|

货号: RP01763

详细信息

种属

Human

表达宿主

Pichia

Calculated MW

12.30 kDa

Observed MW

10-17 kDa

标签

No-tag

内毒素

< 0.1EU/μg

制剂

Lyophilized from a 0.22 μm filtered solution of 20mM NaAc，pH 4.5.

描述

Recombinant Human PDGF-BB Protein is produced by Pichia expression system. The target protein is expressed with sequence (Ser82-Thr190) of human PDGF-BB (Accession #NP_002599.1) fused with a additional amino acid free.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

1.Immobilized PDGF-BB (Catalog: RP01763) at 2 μg/mL (100 μL/well) can bind Human PDGFRB (Catalog: RP00126) with a linear range of 0.1-75.4 ng/mL.2.Recombinant Human PDGF-BB Protein was measured in a cell proliferation assay using BALB/3T3 mouse fibroblasts. The ED50 for this effect is 2.09-8.36 ng/mL, corresponding to a specific activity of 1.20×105~4.78×105 units/mg.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

Platelet-derived growth factor-B (PDGFB) is necessary for normal cardiovascular development. The administration of PDGFB alone normalized tumor vasculature by increasing periendothelial coverage and vascular functionality. Interestingly, this effect exerted by PDGFB was also observed in the presence of DAPT. So PDGFB is able to improve tumor vascularity and allows the anticancer action of DAPT in the tumor.

基因ID

5155

Swiss Prot

P01127

别名

SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2;PDGF subunit B;PDGF-BB;PDGFB

研究领域