产品中心 / 产品类型 / 重组蛋白 / 细胞因子 & 细胞因子受体

Recombinant Human CXCL2/MIP-2 Protein (RP01857)

说明书 联系我们 收藏

Recombinant Human CXCL2/MIP-2 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 20-25 kDa.

All(1)|
货号: RP01857
促销价：   ￥780
抗体定制服务咨询 |
商城订购

详细信息

种属
Human
表达宿主
E.coli
描述
Recombinant Human CXCL2/MIP-2 Protein is produced by E.coli expression system. The target protein is expressed with sequence (Ala35-Asn107) of Human CXCL2/MIP-2 (Accession #NP_002080.1) fused with No tag.
标签
No-Tag
内毒素
<1EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of 20mMTris,500mMNaCl,pH8.0
储存
Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
=
÷

背景信息

CXCL2 is a chemokine induced by endotoxin and serves as an extremely potent chemo-attractant for neutrophils, acting as a crucial inflammatory mediator. CXCL2 could be produced by multiple, different cell types, including macrophages and cancer cells. CXCL2 is involved in cancer metastasis, angiogenesis, and wound healing. The amino acid sequence of human CXCL2 protein has low homology between mouse and rat CXCL2 protein. CXCL2 is 90% identical in amino acid sequence as a related chemokine, CXCL1. The gene for CXCL2 is located on human chromosome 4 in a cluster of other CXC chemokines. CXCL2 binds to the G-protein coupled receptor CXCR2 (IL-8RB) expressed on macrophages, neutrophils, and epithelial cells and its classical function is to act as chemotactic factors attracting neutrophils to sites of injury. In enterocytes, LPS induces CXCL2 expression and promotes migration of neutrophils in a model of platelet-activating factor induced shock and bowel injury. In acute lung injury, CXCR2 ligands, including CXCL1/2/3, have chemotactic effects for polymorphonuclear leukocytes. CXCL2 could provoke a dose-dependent increase of colorectal tumor cell migration in vitro. Further, according to Bachmeier et al., CXCL-1 and −2 silencing could down-regulate several metastasis-promoting genes and inhibit the metastatic potential of breast cancer cells.

基因ID
2920
Swiss Prot
P19875
别名
CXCL2; GRO2; GROB; MIP2A; SCYB2;C-X-C motif chemokine 2; Growth-regulated protein beta; Gro-beta; Macrophage inflammatory protein 2-alpha; MIP2-alpha; Cleaved into: GRO-beta(5-73); GRO-beta-T; Hematopoietic synergistic factor; HSF; SB-251353
研究领域
>