Recombinant Mouse Dkk-3 Protein (RP01894)

Recombinant Mouse Dkk-3 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 45-50kDa.

Recombinant Mouse Dkk-3 inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED₅₀ for this effect is 9.66-38.64 μg/mL, corresponding to a specific activity of 2.59×10¹~1.04×10² units/mg.

All(2)|

货号: RP01894

详细信息

种属

Mouse

表达宿主

HEK293 cells

Calculated MW

37.14 kDa

Observed MW

55-70 kDa

标签

C-His

内毒素

<0.01EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

描述

Recombinant Mouse Dkk-3 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala 22 - Ile 349) of Mouse Dkk-3 (Accession #NP_056629.1) fused with a His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED₅₀ for this effect is 9.66-38.64 μg/mL, corresponding to a specific activity of 2.59×10¹~1.04×10² units/mg.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

DKK3 (dickkopf related protein 3) is a member of the dickkopf-related family consisting of DKK1, DKK2, DKK3 and DKK4. It is a secreted protein, and also known as REIC (Reduced Expansion in Immortalized Cells). The DKK3 protein is proposed to function as a secreted tumor suppressor since it is downregulated in a number of cancer cells and prostate cancer tissue and may be a promising candidate molecule for therapeutic interference. DKK3 protein is also a negative regulator of beta-catenin and its downregulation contribute to an activation of the beta-catenin signaling pathway.

基因ID

50781

Swiss Prot

Q9QUN9

别名

Dkk3;Dickkopf-related protein 3; Dickkopf-3; Dkk-3; mDkk-3