产品和服务 / 产品类型 / 细胞系&裂解液 / KO细胞系

活性验证

Recombinant Mouse Dkk-3 Protein (RP01894)

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Recombinant Mouse Dkk-3 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Recombinant Mouse Dkk-3 inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED50 for this effect is 9.66-38.64 μg/mL, corresponding to a specific activity of 2.59×101~1.04×102 units/mg.

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货号: RP01894
价 格：  620.00
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详细信息

靶点
Dkk3
细胞系
37.14 kDa
突变描述
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.
敲除验证
55-70 kDa
产品组成
Ala 22 - Ile 349
物种
HEK293 cells
保存条件
Store at -20℃
运输条件
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

背景信息

DKK3 (dickkopf related protein 3) is a member of the dickkopf-related family consisting of DKK1, DKK2, DKK3 and DKK4. It is a secreted protein, and also known as REIC (Reduced Expansion in Immortalized Cells). The DKK3 protein is proposed to function as a secreted tumor suppressor since it is downregulated in a number of cancer cells and prostate cancer tissue and may be a promising candidate molecule for therapeutic interference. DKK3 protein is also a negative regulator of beta-catenin and its downregulation contribute to an activation of the beta-catenin signaling pathway.

基因ID
50781
Swiss Prot
Q9QUN9
别名
Dkk3;Dickkopf-related protein 3; Dickkopf-3; Dkk-3; mDkk-3