Recombinant Human CCL25/TECK Protein (RP01945)

说明书收藏

Recombinant Human CCL25/TECK Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 20-25 kDa.

All(1)|

货号: RP01945

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

15.01 kDa

Observed MW

20-25 kDa

标签

C-His

纯度

> 90% by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

描述

Recombinant Human CCL25/TECK Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln24-Leu150）of Human CCL25/TECK(Accession #NP_000726.1&NP_005615.2) fused with a His tag at the C-terminus.

储存

Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifμge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

CCL25, also known as TECK, is a CC chemokine that regulates the trafficking of lymphocytes in the thymus and small intestine. Mature human CCL25 shares 40% amino acid sequence identity with mouse and rat CCL25. CCL25 is produced by stromal cells in the thymus and epithelial cells of the small intestine, particularly the jejunum and ileum. It binds to and induces chemoattraction through CCR9 , and both human and mouse proteins act on human CCR9. CCR9 is expressed on immature pre-T cells and thymocytes. In cancer, functional CCR9 mediates the metastasis of melanoma cells to the small intestine, contributes to the CCL25-dependent migration and invasion of some breast carcinomas , and attracts mesenchymal stromal cells to CCL25-expressing multiple myelomas . CCL25 contributes to the severity of chronic inflammation in rheumatoid arthritis where it attracts CCR9+ monocytes and macrophages, in endometriosis where it promotes the invasiveness of stromal cells, and in atherosclerosis where it contributes to the accumulation of CCR9+ macrophages in arterial plaques.

基因ID

6370

Swiss Prot

O15444

别名

CCL25; SCYA25; TECK;C-C motif chemokine 25; Chemokine TECK; Small-inducible cytokine A25; Thymus-expressed chemokine

研究领域