Recombinant Human Glutathione S-transferase Mu 2/GST4/GSTM2 Protein (RP02122)

说明书联系我们收藏

Recombinant Human GST4/GSTM2 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 25-30 kD.

All(1)|

货号: RP02122

详细信息

种属

Human

表达宿主

E. coli

Calculated MW

27.2 kDa

Observed MW

27 kDa

标签

N-His

纯度

> 97% by SDS-PAGE.

内毒素

Please contact us for more information.

制剂

Lyophilized from a 0.22 μm filtered solution of 50mM Tris, 150mM NaCl, 10mM CaCl2, 0.05% Brig-35, pH 7.5.

描述

Recombinant Human Glutathione S-transferase Mu 2/GST4/GSTM2 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Lys218) of Human Glutathione S-transferase Mu 2/GST4/GSTM2 (Accession #NP_000839.1) fused with a 6×His tag at the N-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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背景信息

Glutathione S-transferase Mu 2, also known as GST class-mu 2, GSTM2-2, and GSTM2, is a cytoplasm protein that belongs to the GST superfamily and Mu family. GSTM2 / GST4 contains one GST C-terminal domain and one GST N-terminal domain. The glutathione S-transferases (GSTs) are a multigene family of enzymes largely involved in the detoxification of chemicals. Eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta, and zeta. Butyrate, an important luminal component produced from the fermentation of dietary fibers, is an efficient inducer of GSTs and especially of GSTM2. Butyrate may act chemoprotective by increasing detoxification capabilities in the colon mucosa.

基因ID

2946

Swiss Prot

P28161

别名

GSTM2;GST4;GSTM;GSTM2-2;GTHMUS

研究领域