产品和服务 / 产品类型 / 重组蛋白 / 其他蛋白

Recombinant Mouse Calcitonin/CALCA Protein (RP02196)

文献引用 (1) 说明书 收藏

Mouse CALCA/CGRP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Mouse CALCA/CGRP is greater than 95% as determined by SEC-HPLC.

All(2)|
货号: RP02196

促销价：   ￥4000
货    期：在线咨询货期
抗体定制服务咨询 |
扫码下单
享受积分

详细信息

种属
Mouse
表达宿主
HEK293 Cells
Calculated MW
36.9 kDa
Observed MW
42-46 kDa
标签
C-hFc
纯度
> 95% as determined by HPLC.
内毒素
<1EU/μg
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
描述
Recombinant Mouse Calcitonin/CALCA Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Val26-Asp119) of mouse  Calcitonin/CALCA (Accession #NP_001029126.1) fused with  hFc tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
=
÷

客户数据及评论 (0)

折叠内容

文献引用 (1)

折叠内容

Publishing research using RP02196? Please let us know so that we can cite the reference in this datasheet.

背景信息

Specific fragments of methylation changes in the target gene (Calca) were identified by IGV analysis. CGRP was applied to compare the effects on ASCs-T2DM morphology via phalloidin staining, proliferation through CCK-8 assay, and osteogenic differentiation with osteogenic staining, qPCR, and repair of calvarial defect. Furthermore, 5-azacytidine (5-az) was used to intervene ASCs-T2DM to verify the relationship between the methylation level of the target fragment and expression of Calca.

基因ID
12310
Swiss Prot
Q99JA0
别名
Calcitonin; CCP; PDN-21; CALCA; CALC1; CGRP; CGRP-I; CGRP1; CT; KC; PCT; Procalcitonin; Calcitonin 1; Katacalcin
    >