SARS-COV-2 Spike S Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Immobilized SARS-COV-2 Spike S Trimer,His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human ACE2,hFc Tag with the EC50 of 47.3/37.8/37.0ng/ml determined by ELISA.
Immobilized SARS-COV-2 Spike S Trimer,His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human ACE2,hFc Tag with the EC50 of 47.3ng/ml determined by ELISA.
The purity of SARS-COV-2 Spike S Trimer is greater than 95% as determined by SEC-HPLC.
Recombinant SARS-COV-2 Spike S Trimer Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Val16-Glu1188) of SARS-COV-2 Spike S Trimer fused with His tag and Avi tag at the C-terminal.
标签
His tag and Avi tag at the C-terminal
纯度
> 95% by Tris-Bis PAGE;> 95% by SEC-HPLC
内毒素
< 1 EU/μg of the protein by LAL method.
生物活性
Immobilized SARS-COV-2 Spike S Trimer,His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human ACE2,hFc Tag with the EC50 of 47.3/37.8/37.0ng/ml determined by ELISA. Immobilized SARS-COV-2 Spike S Trimer,His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human ACE2,hFc Tag with the EC50 of 47.3ng/ml determined by ELISA.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
储存
Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月; 复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
复溶
Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。