Recombinant Cynomolgus B7-H6/NCR3LG1 Protein (RP02646)

说明书收藏

Cynomolgus B7-H6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Cynomolgus B7-H6 is greater than 95% as determined by SEC-HPLC.

Human NKp30, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus B7-H6, His Tag with an affinity constant of 0.662 μM as determined in SPR assay (Biacore T200).

All(3)|

货号: RP02646

详细信息

种属

Cynomolgus

表达宿主

HEK293 cells

Calculated MW

27.6 kDa

Observed MW

40-52 kDa

标签

C-His

纯度

> 95% as determined by Tris-Bis PAGE；> 95% as determined by HPLC

内毒素

Less than 1EU per μg by the LAL method.

描述

Recombinant Cynomolgus B7-H6/NCR3LG1 Protein is expressed from Expi293 with His tag at the C-terminal.；It contains Asp25-Asp259.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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背景信息

B7-H6 is a glycosylated member of the B7 family of immune co?stimulatory proteins. which is a ligand for the NK cell activating receptor NKp30, was targeted to create a CAR that targets multiple tumor types. B7H6 is expressed on various primary human tumors, including leukemia, lymphoma and gastrointestinal stromal tumors, but it is not constitutively expressed on normal tissues.

基因ID

102121659

Swiss Prot

XP_005578557.1

别名

B7H6; B7-H6; DKFZp686I21167; DKFZp686O24166; NCR3LG1; B7 Homolog 6