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Recombinant Human IL-17RB Protein (RP02679)

Human IL-17RB on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Human IL-17RB is greater than 95% as determined by SEC-HPLC.

Immobilized Human IL-17RB, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-17RB Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.

Human IL-17RB, His Tag immobilized on CM5 Chip can bind Human IL-25, His Tag with an affinity constant of 0.81 nM as determined in SPR assay (Biacore T200).

Immobilized Human IL-17RB, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-25, His Tag with the EC50 of 58.5ng/ml determined by ELISA.

All(5)|
货号: RP02679
促销价:   ¥3000
货    期:在线咨询货期
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详细信息

种属
Human
表达宿主
HEK293 cells
Calculated MW
31.4 kDa
Observed MW
45-60 kDa
标签
C-His
纯度
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
内毒素
Less than 1EU per μg by the LAL method.
描述
Recombinant Human IL-17RB Protein is expressed from Expi293 with His tag at the C-terminal.;It contains Arg18-Per292.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
复溶
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度,快速计算您需要添加溶液的体积吧!
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背景信息

Among inflammatory mediators, a growing body of evidence emphasizes the contribution of the interleukin 17 (IL-17) cytokine family in malignant diseases. Besides IL-17A, the prototypic member of the IL-17 family, several experimental findings strongly support the role of the IL-17B/IL-17 receptor B (IL-17RB) pathway in tumorigenesis and resistance to anticancer therapies. In mouse models, IL-17B signaling through IL-17RB directly promotes cancer cell survival, proliferation, and migration, and induces resistance to conventional chemotherapeutic agents.