Recombinant Human P53 R175H (HLA-A*02:01) Complex Protein (RP02688)

说明书收藏

Human P53 R175H (HLA-A*02:01) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Human P53 R175H (HLA-A*02:01) is greater than 95% as determined by SEC-HPLC.

Immobilized Anti-P53 R175H (HLA-A*02:01) Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human P53 R175H (HLA-A*02:01) , His Tag with the EC₅₀ of 0.19μg/ml determined by ELISA.

All(3)|

货号: RP02688

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

50.5 kDa

Observed MW

51-60 kDa

标签

C-His&Avi

纯度

> 95% as determined by Tris-Bis PAGE；> 95% as determined by HPLC

内毒素

Less than 1EU per μg by the LAL method.

描述

Recombinant Human P53 R175H (HLA-A*02:01) Complex Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.；It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and HMTEVVRHC peptide.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

p53 is a tumor suppressor protein. Under stressful conditions, p53 tightly regulates cell growth by promoting apoptosis and DNA repair. When p53 becomes mutated, it loses its function, resulting in abnormal cell proliferation and tumor progression. Depending on the p53 mutation, it has been shown to form aggregates leading to negative gain of function of the protein.p53 mutant associated aggregation has been observed in several cancer tissues and has been shown to promote tumor growth.

Swiss Prot

A0A140T913(HLA-A*02:01)&P61769(B2M)& HMTEVVRHC

别名

MHC; HLA-A; P53; TP53; Antigen NY-CO-13; BCC7; FLJ92943; LFS1; TRP53