Biotinylated Recombinant Mouse GARP&Latent TGF beta 1 Complex Protein (RP02766B)

Biotinylated Mouse GARP&Latent TGF beta 1 Complex on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Mouse GARP&Latent TGF beta 1 Complex is greater than 95% as determined by SEC-HPLC.

Immobilized Mouse ITGAV&ITGB6, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse GARP&Latent TGF beta 1 Complex, His Tag with the EC₅₀ of 0.12μg/ml determined by ELISA.

All(3)|

货号: RP02766B

详细信息

种属

Mouse

表达宿主

HEK293 cells

Calculated MW

66.6 kDa (GARP), 44.2 kDa (Latent TGF beta 1)

Observed MW

72-76 kDa (GARP), 13, 45-47 kDa (Latent TGF beta 1)

标签

N-His&Avi

纯度

> 95% by Tris-Bis PAGE;> 95% by SEC-HPLC

内毒素

< 1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

描述

Biotinylated Recombinant Mouse GARP&Latent TGF beta 1 Complex Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Ala18-Leu628(GARP)&Leu30-Ser390(Latent TGF beta 1)) of Mouse GARP&Latent TGF beta 1 fused with a His and Avi tag at the N-terminal.

储存

Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.

基因ID

21803&434215

Swiss Prot

G3XA59(GARP)&P04202(Latent TGF beta 1)

别名

LRRC32; GARP; LAP; TGF-beta-1; LRRC32&TGF-beta 1; LRRC32&TGFB1

研究领域