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Recombinant Human Thrombopoietin R/MPL Protein (RP02785)

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Recombinant Human Thrombopoietin R/MPL Protein was determined by SDS-PAGE with Coomassie Blue, showing bands at 68-70 kDa.

The purity of Human Thrombopoietin R/MPL is greater than 95% as determined by SEC-HPLC.

Immobilized Human c-MPL, His Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-c-MPL Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA.

All(3)|
货号: RP02785
促销价：   ￥3450
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详细信息

种属
Human
表达宿主
HEK293 cells
描述
Recombinant Human Thrombopoietin R/MPL Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln26-Trp491) of human Thrombopoietin R/MPL (Accession #NP_005364.1) fused with a 6×His tag at the C-terminus.
标签
C-His
纯度
> 95% as determined by HPLC
内毒素
<1EU/μg
生物活性
Immobilized Human c-MPL, His Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-c-MPL Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
储存
Store the lyophilized protein at -20°C to -80°C for 12 months.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

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背景信息

The c-mpl gene encodes a member of the hematopoietic cytokine receptor superfamily. This gene was discovered through the study of a murine retrovirus which induces an acute myeloproliferative syndrome in mice.MPLV (for myeloproliferative leukemia virus) has transduced a truncated and constitutively activated form of the c-mpl receptor chain. High levels of c-mpl expression were detected in 45% of acute myeloid leukemia (AML). No correlation was found between c-mpl expression and the French-American-British classification subtype of AML.

基因ID
4352
Swiss Prot
P40238
别名
TPO-R; CD110; MPL; c-Mpl; MPLV; K12
研究领域
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