Recombinant Human Thrombopoietin R/MPL/CD110 Protein (RP02785)

Recombinant Human Thrombopoietin R/MPL Protein was determined by SDS-PAGE with Coomassie Blue, showing bands at 68-70 kDa.

The purity of Human Thrombopoietin R/MPL is greater than 95% as determined by SEC-HPLC.

Immobilized Human c-MPL, His Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-c-MPL Antibody, hFc Tag with the EC₅₀ of 9.1ng/ml determined by ELISA.

All(3)|

货号: RP02785

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

53.6 kDa

Observed MW

68-70 kDa

标签

C-His

纯度

> 95% as determined by HPLC

内毒素

<1EU/μg

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

描述

Recombinant Human Thrombopoietin R/MPL/CD110 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln26-Trp491) of human Thrombopoietin R/MPL (Accession #NP_005364.1) fused with a 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Immobilized Human c-MPL, His Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-c-MPL Antibody, hFc Tag with the EC₅₀ of 9.1ng/ml determined by ELISA.

复溶

Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

客户数据及评论 (0)

折叠内容

背景信息

The c-mpl gene encodes a member of the hematopoietic cytokine receptor superfamily. This gene was discovered through the study of a murine retrovirus which induces an acute myeloproliferative syndrome in mice.MPLV (for myeloproliferative leukemia virus) has transduced a truncated and constitutively activated form of the c-mpl receptor chain. High levels of c-mpl expression were detected in 45% of acute myeloid leukemia (AML). No correlation was found between c-mpl expression and the French-American-British classification subtype of AML.

基因ID

4352

Swiss Prot

P40238

别名

TPO-R; CD110; MPL; c-Mpl; MPLV; K12

研究领域