Recombinant Human Insulin Protein (RP02930LQ)

说明书收藏

Recombinant Human Insulin Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 7 kDa.

All(1)|

货号: RP02930LQ

详细信息

种属

Human

表达宿主

Yeast

Calculated MW

7.1 kDa

Observed MW

6 kDa

标签

No tag

纯度

> 95% by SDS-PAGE.

内毒素

Please contact us for more information.

制剂

Supplied as a 0.22 μm filtered solution in PBS, pH 7.4. Contact us for customized product form or formulation.

描述

Recombinant Human Insulin Protein is produced by Yeast expression system. The target protein is expressed with sequence ((Phe25-Lys53)AAK(Gly90-Asn110)) of human Insulin (Accession #NP_000198.1) fused with no additional amino acid.

储存

Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells.The ED₅₀ for this effect is typically 0.2-1 μg/mL.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

INS (Insulin) is a Protein Coding gene. This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. The binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Diseases associated with INS include Hyperproinsulinemia and Maturity-Onset Diabetes Of The Young, Type 10. A multitude of mutant alleles with phenotypic effects has been identified, including insulin-dependent diabetes mellitus, permanent neonatal diabetes mellitus, maturity-onset diabetes of the young type 10, and hyperproinsulinemia.

基因ID

3630

Swiss Prot

P01308

别名

IDDM;IDDM1;IDDM2;ILPR;IRDN;MODY10;INS;Insulin;insulin

研究领域