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Recombinant Human SFRP2 Protein (RP02970LQ)

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Recombinant Human SFRP2 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 34-38 kDa.

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货号: RP02970LQ
促销价：   ￥3200
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详细信息

种属
Human
表达宿主
HEK293 cells
描述
Recombinant Human SFRP2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu25-Cys295) of human SFRP2 (Accession #NP_003004.1) fused with a 6×His tag at the C-terminus.
标签
C-His
纯度
> 95% by SDS-PAGE.
内毒素
<1EU/μg
制剂
Lyophilized from a 0.22 μm filtered solution of 20mM PBS, 100mM L-arginine (pH 7.4). Contact us for customized product form or formulation.
储存
Store at -20℃.This product is stable at -20℃ to -80 ℃ up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

As biomarkers, DNA methylation is used to detect colorectal cancer (CRC) and make assessment of CRC prognosis. The published findings showed the association between the methylation of SFRP1, SFRP2, and WIF1, located in the Wnt signaling pathway, and the prognosis of CRC were not consistent. SFRP1, SFRP2, and WIF1 were frequently hypermethylated in CRC tumor tissues. It was apparent that the promoter hypermethylation of SFRP2 and co-hypermethylation of SFRP1 and SFRP2 might be considered as independent prognostic predictors for survival advantage of postoperative CRC patients.

基因ID
6423
Swiss Prot
Q96HF1
别名
sFRP-2; SARP-1; Sarp1; Sdf5; Sfrp2; AI851596
研究领域
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