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活性验证

Recombinant Human KIF18A Protein (RP02971LQ)

Recombinant Human KIF18A Protein was determined by SDS-PAGE with Coomassie Blue, showing bands at 44 kDa.

KIF18A activity test using ADP-Glo method.The reaction was performed by incubating the KIF18A protein and ATP at 25℃ for 60 min, adding ADP-Glo Reagent incubating for 40 min subsequently, then reading luminescence signal with BMG.

KIF18A activity test using ADP-Glo method.The reaction was performed by incubating the KIF18A protein and ATP at 25℃ for 60 min, adding ADP-Glo Reagent incubating for 40 min subsequently, then reading luminescence signal with BMG.

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货号: RP02971LQ
促销价:   ¥2500
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详细信息

种属
Human
表达宿主
E. coli
描述
Recombinant human KIF18A protein with N-terminal 6x his tag +TEV cleavage site was purified by Ni-NTA affinity and followed by SEC chromatography. The KIF18A protein showed high activity in the ATPase activity test.
标签
N-6His
纯度
> 95% by SDS-PAGE
生物活性
KIF18A activity test using ADP-Glo method.
制剂
Supplied as a 0.22 μm filtered solution in 20mM Tris, 150mM NaCl, 1mM DTT, 10% glycerol, pH7.4
储存
This product is stable at ≤ -70°C for up to 1 year from the date of receipt.
For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。

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请在蛋白复溶计算器中输入蛋白总质量和所需终浓度,快速计算您需要添加溶液的体积吧!
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背景信息

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