Recombinant Human KIF18A Protein (RP02971LQ)

Recombinant Human KIF18A Protein was determined by SDS-PAGE with Coomassie Blue, showing bands at 44 kDa.

KIF18A activity test using ADP-Glo method.The reaction was performed by incubating the KIF18A protein and ATP at 25℃ for 60 min, adding ADP-Glo Reagent incubating for 40 min subsequently, then reading luminescence signal with BMG.

All(3)|

货号: RP02971LQ

详细信息

种属

Human

表达宿主

E. coli

Calculated MW

44 kDa

Observed MW

45-47 kDa

标签

N-6His

纯度

> 95% by SDS-PAGE

制剂

Supplied as a 0.22 μm filtered solution in 20mM Tris, 150mM NaCl, 1mM DTT, 10% glycerol, pH7.4

描述

Recombinant human KIF18A protein with N-terminal 6x his tag +TEV cleavage site was purified by Ni-NTA affinity and followed by SEC chromatography. The KIF18A protein showed high activity in the ATPase activity test.

储存

Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

KIF18A activity test using ADP-Glo method.