Recombinant Human PARP2 Protein was determined by SDS-PAGE with Coomassie Blue, showing bands at 92.4 kDa.
The PARP2 activity was assayed with ELISA technology. The reaction was performed by:(1)Histone protein was coated on a 96-well plate.(2)A biotinylated NAD+ mix is incubated with varying different concentration of the PARP2 enzyme and an activated DNA. (3)The plate was treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence. Finally, measured the signal using a chemiluminescence reader
The PARP2 activity was assayed with ELISA technology. The reaction was performed by:(1)Histone protein was coated on a 96-well plate.(2)A biotinylated NAD+ mix is incubated with varying different concentration of the PARP2 enzyme and an activated DNA.(3)The plate was treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence. Finally, measured the signal using a chemiluminescence reader
