Recombinant Human POLQ (1819-2590) Protein (RP02982LQ)

Recombinant Human POLQ (1819-2590) Protein was determined by SDS-PAGE with Coomassie Blue, showing bands at 88.6 kDa.

POLQ (1819-2590) polymerase activity test using FI method. The POLQ (1819-2590) polymerase activity was assayed with PicoGreen technology. The reaction was performed by incubating the POLQ (1819-2590) protein, fluorescence-labeled dsDNA and PicoGreen at 25C for 90min, then reading 485nm and 520nm fluorescence signal with BMG.

All(3)|

货号: RP02982LQ

详细信息

种属

Human

表达宿主

Baculovirus-Infected Sf9 Cells

Calculated MW

88.6 kDa

Observed MW

90-100 kDa

标签

N-6His

纯度

> 95% by SDS-PAGE

制剂

Supplied as a 0.22 μm filtered solution in 20 mM Tris, 500 mM NaCl, 2mM B-ME, 5% glycerol, pH 8.5

描述

Recombinant human POLQ (1819-2590) protein with N-terminal 6x his tag +TEV cleavage site was purified by Ni-NTA affinity and followed by SEC chromatography. The POLQ (1819-2590) protein showed high activity in TR-FRET and ADP-Glo assay.

储存

Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

1.POLQ (1819-2590) polymerase activity test using TR-FRET method.
2.POLQ (1819-2590) polymerase activity test using FI method.