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活性验证

Recombinant Human WRN (500-946) Protein (RP02984LQ)

Recombinant Human WRN (500-946) Protein was determined by SDS-PAGE with Coomassie Blue, showing bands at 53 kDa.

WRN activity test using ADP-Glo method.The WRN (500-946) activity was assayed with ADP-Glo technology. The reaction was performed by incubating the WRN protein, ATP and dsDNA at 25℃ for 60 min, then reading luminescence signal with BMG.

WRN activity test using ADP-Glo method.The WRN (500-946) activity was assayed with ADP-Glo technology. The reaction was performed by incubating the WRN protein, ATP and dsDNA at 25℃ for 60 min, then reading luminescence signal with BMG.

WRN (500-946) activity test using FI method. The WRN (500-946) unwinding activity was assayed with FI technology. The reaction was performed by incubating the WRN protein, Cy5-labeled dsDNA at 25℃ for 60 min, then reading EX620/EM680 fluorescence signal with BMG.

WRN (500-946) activity test using FI method. The WRN (500-946) unwinding activity was assayed with FI technology. The reaction was performed by incubating the WRN protein, Cy5-labeled dsDNA at 25℃ for 60 min, then reading EX620/EM680 fluorescence signal with BMG.

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货号: RP02984LQ
促销价:   ¥3000
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详细信息

种属
Human
表达宿主
Baculovirus-Infected Sf9 Cells
描述
Recombinant human WRN (500-946) protein with N-terminal 6x his tag +TEV cleavage site was purified by Ni-NTA affinity and followed by SEC chromatography. The WRN protein showed high activity in TR-FRET and ADP-Glo assay
标签
N-6His
纯度
> 95% by SDS-PAGE
生物活性
1.WRN activity test using ADP-Glo method.
2.WRN (500-946) activity test using FI method.
制剂
Supplied as a 0.22 μm filtered solution in 100 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol
储存
This product is stable at ≤ -70°C for up to 1 year from the date of receipt.
For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。

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请在蛋白复溶计算器中输入蛋白总质量和所需终浓度,快速计算您需要添加溶液的体积吧!
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