Recombinant Human WRN (500-946) Protein was determined by SDS-PAGE with Coomassie Blue, showing bands at 53 kDa.
WRN activity test using ADP-Glo method.The WRN (500-946) activity was assayed with ADP-Glo technology. The reaction was performed by incubating the WRN protein, ATP and dsDNA at 25℃ for 60 min, then reading luminescence signal with BMG.
WRN activity test using ADP-Glo method.The WRN (500-946) activity was assayed with ADP-Glo technology. The reaction was performed by incubating the WRN protein, ATP and dsDNA at 25℃ for 60 min, then reading luminescence signal with BMG.
WRN (500-946) activity test using FI method. The WRN (500-946) unwinding activity was assayed with FI technology. The reaction was performed by incubating the WRN protein, Cy5-labeled dsDNA at 25℃ for 60 min, then reading EX620/EM680 fluorescence signal with BMG.
WRN (500-946) activity test using FI method. The WRN (500-946) unwinding activity was assayed with FI technology. The reaction was performed by incubating the WRN protein, Cy5-labeled dsDNA at 25℃ for 60 min, then reading EX620/EM680 fluorescence signal with BMG.
Recombinant human WRN (500-946) protein with N-terminal 6x his tag +TEV cleavage site was purified by Ni-NTA affinity and followed by SEC chromatography. The WRN protein showed high activity in TR-FRET and ADP-Glo assay
标签
N-6His
纯度
> 95% by SDS-PAGE
生物活性
1.WRN activity test using ADP-Glo method. 2.WRN (500-946) activity test using FI method.
制剂
Supplied as a 0.22 μm filtered solution in 100 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol
储存
This product is stable at ≤ -70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月; 复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。