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Recombinant Mouse Amphiregulin/AREG Protein (RP03167)

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Recombinant Mouse Amphiregulin/AREG Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 45-50 kDa.

The purity of Mouse AREG is greater than 95% as determined by SEC-HPLC.

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货号: RP03167
促销价：   ￥3000
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详细信息

种属
Mouse
表达宿主
HEK293 cells
描述
Recombinant Mouse Amphiregulin/AREG Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val100-Ala248) of Mouse Amphiregulin/AREG (Accession #NP_033834.1) fused with hFc tag at the N-terminus.
标签
N-hFc
纯度
> 95% as determined by HPLC
内毒素
< 1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.
储存
Store the lyophilized protein at -20°C to -80 °C for 12 months.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

Amphiregulin (AREG) is a ligand of the epidermal growth factor receptor (EGFR), a widely expressed transmembrane tyrosine kinase. AREG is synthesized as a membrane-anchored precursor protein that can engage in juxtacrine signaling on adjacent cells. Alternatively, after proteolytic processing by cell membrane proteases, mainly TACE/ADAM17, AREG is secreted and behaves as an autocrine or paracrine factor.

基因ID
11839
Swiss Prot
P31955
别名
AREG;AR;AREGB;CRDGF;SDGF;amphiregulin
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