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Recombinant Human Inhibitor of growth protein 4/ING4 Protein (RP03219)

Recombinant Human ING4 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 25-30 kDa.

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货号: RP03219
促销价:   ¥4500
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详细信息

种属
Human
表达宿主
E.coli
Calculated MW
30.4 KDa
Observed MW
25-30 kDa
标签
N-His
纯度
> 85% by SDS-PAGE.
内毒素
Please contact us for more information.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.
描述
Recombinant Human Inhibitor of growth protein 4/ING4 Protein is produced by E. coli expression system. The target protein is expressed with sequence(Met1-Lys249) of Human Inhibitor of growth protein 4 /ING4 (Accession #Q9UNL4-1) fused with His tag at the N-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度,快速计算您需要添加溶液的体积吧!
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背景信息

ING4 is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. ING4 contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. ING4 protein can bind TP53 and EP3/p3, a component of the histone acetyltransferase complex, suggesting its involvement in the TP53-dependent regulatory pathway. ING4 is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation, it may function in DNA replication. ING4 may also inhibit tumor progression by modulating the transcriptional output of signaling pathways that regulate cell proliferation.

基因ID
Swiss Prot
别名
ING4; my036; p29ING4
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