Recombinant Human Heat shock cognate 71 kDa protein/HSC70 Protein (RP03267)

Recombinant Human HSC70 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at approximately 60-70 kDa.

All(1)|

货号: RP03267

详细信息

种属

Human

表达宿主

E.coli

Calculated MW

72.4 kDa

Observed MW

60-70 kDa

标签

N-His

纯度

> 90% by SDS-PAGE.

内毒素

Please contact us for more information.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, 10% glycerol, pH 7.5. Contact us for customized product form or formulation.

描述

Recombinant Human HSC70 Protein is produced by E.coli expression system. The target protein is expressed with sequence (Met1-Asp646) of human HSC70 (Accession #P11142-1) fused with His tag at the N-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

HSC70, also known as HSPA8, is a member of the heat shock protein family. It is homologous to other heat shock proteins. The heat shock protein 70 family is comprised of both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. HSPA8 belongs to the heat-shock cognate subgroup. Members of the human heat-shock protein multigene family have several highly conserved proteins with structural and functional properties in common but vary in the extent of their inducibility in response to metabolic stress. HSPA8 is constitutively expressed and performs functions related to normal cellular processes. This protein binds to nascent polypeptides to facilitate correct protein folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during the transport of membrane components through the cell.

基因ID

3312

Swiss Prot

P11142-1

别名

Heat shock cognate 71 kDa protein; Heat shock 70 kDa protein 8; HSPA8; HSC70; HSP73; HSPA10; Lipopolysaccharide-associated protein 1; LPS-associated protein 1; LAP-1; HEL-33; NIP71

研究领域