Recombinant Human CDK12&Cyclin K Protein was resolved with SDS-PAGE under reducing (Lane 1) and non-reducing (Lane 2) conditions.
The activity of CDK12/Cyclin K is based on the MSA technology, and the content and ratio of the substrate and the product are directly separated and detected in real time and dynamically by the different migration rates of the substrate and the product after the enzymatic reaction.
CDK12 also known as cell cycle-dependent protein kinase 12 is a cell cycle-dependent protein kinase that functions as a transcription-related kinase. It forms a heterodimeric complex with Cyclin K, which phosphorylates the carboxyl-terminal domain of RNA polymerase II (RNA Pol II) to regulate gene expression. CDK12 is also the most frequently mutated of all CDKs, reaching up to 5%, and its genomic alterations have been identified in cancers, such as prostate cancer, breast cancer, and colorectal cancer.
