Recombinant Human CDK13&Cyclin K Protein was resolved with SDS-PAGE under reducing (Lane 1) and non-reducing (Lane 2) conditions.
The activity of CDK13/Cyclin K is based on the MSA technology, and the content and ratio of the substrate and the product are directly separated and detected in real time and dynamically by the different migration rates of the substrate and the product after the enzymatic reaction.
CDK13 also known as cyclin dependent kinase 13 is a member of the cyclin-dependent serine/threonine protein kinase family. It contains a C-terminal extension helix composed of a polybasic cluster and a DCHEL motif that interacts with the bound ATP. Cyclin K has been shown to interact with multiple CDKs including CDK9 and latest CDK12 and CDK13. Roles include helping to phosphorylate C-terminal domains of subunits of RNAP2. CDK13/Cyclin K as the latest members of RNA polymerase II (RNA Pol II) C-terminal domain (CTD) kinases phosphorylates both Ser5 and Ser2 of the RNA Pol II CTD with a preference for Ser7 pre-phosphorylations at a C-terminal position.
