Recombinant Human CRIK/CIT/STK21 Protein was resolved with SDS-PAGE under reducing (Lane 1) and non-reducing (Lane 2) conditions.
The activity of CIT is based on the ADP-Glo technology, and the ADP-GLO kinase activity assay quantifies kinase activity by measuring the conversion of ATP to ADP catalyzed by the kinase. Specific reagents are used to convert the ADP in the reaction back to ATP, resulting in the production of a luminescent signal.
Citron Rho-interacting kinase (CRIK) is encoded by the CIT gene. Citron kinase, which resembles the ROCK family of kinases and by comparison to it, is therefore a multiple domain protein containing an N-terminal kinase domain, an internal coiled-coil (CC) domain with Rho/Rac interacting site, and a C-terminal region consisting of a Zn finger, a pleckstrin homology (PH) domain, a Citron homology domain (CNH), a putative SH3 binding domain, and a PDZ-targeting motif. Citron kinase is believed to act in cytokinesis and is important to keep proper structure of the midbody which holds the intercellular bridge microtubules between the two daughter cells and is thus required for successful transition from constriction to abscission.
