Recombinant Serratia marcescens Nuclease was determined by SDS-PAGE with Coomassie Blue, showing a band at 35-40kDa.
One unit (U) is defined as the amount of enzyme required to change the absorption value of △A260 by 1.0 (equivalent to complete digestion of 37 μg of salmon essence DNA into oligonucleotides) in 30 min at 37°C, pH 8.0 reaction conditions. The specific activity of Serratia marcescens nuclease is > 1000 U/μL.
Recombinant Serratia marcescens Nuclease (0.1U) can effectively degrade 5 μg plasmid DNA at 37℃ for 30 min. The reaction buffer is: 10 mM MgCl2, 0.1 mg/mL BSA, 50 mM Tris-HCl, pH8.5.
Catalyzes the hydrolysis of both DNA and RNA, double- or single-stranded, at the 3'position of the phosphodiester bond to produce 5'-phosphorylated mono-, di-, tri- and tetranucleotides. DNA is a slightly better substrate than RNA.
