Recombinant Tobacco etch virus TEV Protease (RPT0011LQ)

30 μg protein substrate were kept with different units of recombinant TEV Protease in 50 mM Tris, pH 8.0, 0.1 mM EDTA, 1 mM DTT at 30℃ for 1 hour. The volume of each reaction system was 200 μL. After reaction, 20 μL of each reaction was checked by SDS-PAGE.

All(1)|

货号: RPT0011LQ

详细信息

种属

Tobacco etch virus (TEV)

表达宿主

E. coli

Calculated MW

27.59 kDa

Observed MW

28 kDa

标签

N-His

内毒素

Please contact us for more information.

制剂

Supplied as a 0.22 μm filtered solution in 50mM Tris 100mM NaCl 2mM DTT 50% Glycerol pH8.0.

描述

Recombinant Tobacco etch virus TEV Protease is produced by E. coli expression system. The target protein is expressed with sequence (Gly2038-Asn2273) of tobacco etch virus TEV (Accession #NP_062908.1) fused with a 6×His tag at the N-terminus.

储存

Store at -20℃.This product is stable at ≤ -20℃ for up to 1 year from the date of receipt.
For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

30 μg protein substrate were kept with different units of recombinant TEV Protease in 50 mM Tris, pH 8.0, 0.1 mM EDTA, 1 mM DTT at 30℃ for 1 hour. The volume of each reaction system was 200 μL. After reaction, 20 μL of each reaction was checked by SDS-PAGE.