纯度
≥ 90 % as determined by SDS-PAGE.
内毒素
< 0.5 EU/μg of the protein by LAL method.
制剂
Supplied as 0.2 μm filtered solution in PBS, pH7.4 with glycerol as protectant.
储存
Store at -70℃. The product MUST be stored at -70°C or lower upon receipt;-70°C for 3 months under sterile conditions.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
生物活性
Human Laminin 521 Protein, premium grade (Cat. No. RPT0020LQ) immobilized on CM5 Chip can bind Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free with an affinity constant of 2.55 nM as determined in a SPR assay .
请在蛋白复溶计算器中输入蛋白总质量和所需终浓度,快速计算您需要添加溶液的体积吧!
Laminin 521, a heterotrimeric glycoprotein composed of one alpha 5, one beta 2 and one gamma 1 chain, is a defined matrix for feeder-free culture of human embryonic (hES) and induced pluripotent (hiPS) stem cells. When used in combination with defined maintenance media, Laminin 521 reliably facilitates self-renewal of human hES and hiPS cells without spontaneous differentiation.