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表观修饰性抗体

在哺乳动物基因组中，一个核小体由H2A、H2B、H3、H4各两分子组成的八聚体和200bp左右的DNA以及一个分子的组蛋白H1组成。游离在外的组蛋白N端会在相关酶作用下发生甲基化、乙酰化、磷酸化、腺苷酸化、泛素化、ADP核糖基化等多种修饰，这些修饰都会影响基因的转录活性。研究最热门的是组蛋白的甲基化修饰，其次是乙酰化修饰。组蛋白甲基化是由组蛋白甲基转移酶（Histonemethyl Transferase，HMT）催化完成，可发生在组蛋白的赖氨酸(K)和精氨酸(R)残基上，赖氨酸残基能够发生单、双、三甲基化，而精氨酸残基能够发生单甲基化、对称和不对称双甲基化。组蛋白乙酰化则是由乙酰转移酶介导，人类基因组编码有六十多种组蛋白乙酰转移酶，催化组蛋白的赖氨酸残基上发生单乙酰化。

组蛋白八聚体结构示意图

组蛋白常见修饰位点分布

ABclonal组蛋白修饰性靶点抗体

H3K4me1 H3K4me2 H3K4me3 H3K9me1
H3K9me2 H3K9me3 H3K14me1 H3K14me2
H3K14me3 H3K27me1 H3K27me2 H3K27me3
H3K36me1 H3K36me2 H3K36me3 H3K56me3
H3K64me3 H3K79me1 H3K79me2 H3K79me3
H3R2me1 H3R2me2a H3R2me2s H3R8me1
H3R8me2a H3R8me2s aH3R17me1 H3R17me2a
H3R17me2s H3R26me1 H3R26me2a H3R26me2s
H4K20me1 H4K20me2 H4K20me3 H4R3me1
H4R3me2a H4R3me2S pan-Kme2 pan-ac

ABclonal表观修饰性抗体优势

特异性高：抗体采用交叉纯化，短肽纯化，多肽矩阵检测抗体特异性。

严格QC：表观修饰性抗体检测方案—多物种（H M R），多应用（DB WB IHC IF ChIP），出库前全部经过内部严格QC检测，极大程度满足客户需求。

抗体Kit：ABclonal表观抗体全部自主研发，可根据功能性研究对抗体进行组装，提供系统性解决方案。

ChIP-Seq服务：ABclonal不仅能够提供ChIP级抗体，同时能够提供 ChIP 实验、ChIP-qPCR 检测、高通量测序文库构建、生物信息学数据分析等全套服务。

新靶点合作开发：科学家鉴定出新的修饰位点或者发现新的修饰类型，ABclonal公司可以合作开发，鉴定成功，客户可购买成品。

代表性抗体展示

Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K9 antibody (A2360) at 1:1000 dilution.

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K9 antibody (A2360).

Immunohistochemistry of paraffin-embedded Rat brain using TriMethyl-Histone H3-K9 antibody (A2360) at dilution of 1:100 (40x lens).

Chromatin immunoprecipitation analysis extracts of 293T cells, using TriMethyl-Histone H3-K9 antibody (A2360) and rabbit IgG. P1 and P2 were located on EBAG9 gene. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K27 antibody (A2363) at 1:1000 dilution.

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody (A2363).

Immunofluorescence analysis of HeLacells using TriMethyl-Histone H3-K27 antibody (Red) and BrdU antibody (A1482) (Green).Blue: DAPI for nuclear staining.

Chromatin immunoprecipitation analysis extracts of 293T cells, using TriMethyl-Histone H3-K27 antibody (A2363) and rabbit IgG. P1 and P2 were located on ANO2 gene. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H4-K20 antibody (A2372) at 1:1000 dilution.

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H4-K20 antibody (A2372).

Immunohistochemistry of paraffin-embedded rat testis using TriMethyl-Histone H4-K20 antibody (A2372) at dilution of 1:200 (40x lens).